Doherty Erin A S, Meagher Robert J, Albarghouthi Methal N, Barron Annelise E
Department of Chemical Engineering, Northwestern University, Evanston, IL 60208, USA.
Electrophoresis. 2003 Jan;24(1-2):34-54. doi: 10.1002/elps.200390029.
The necessity for microchannel wall coatings in capillary and chip-based electrophoretic analysis of biomolecules is well understood. The regulation or elimination of EOF and the prevention of analyte adsorption is essential for the rapid, efficient separation of proteins and DNA within microchannels. Microchannel wall coatings and other wall modifications are especially critical for protein separations, both in fused-silica capillaries, and in glass or polymeric microfluidic devices. In this review, we present a discussion of recent advances in microchannel wall coatings of three major classes--covalently linked polymeric coatings, physically adsorbed polymeric coatings, and small molecule additives. We also briefly review modifications useful for polymeric microfluidic devices. Within each category of wall coatings, we discuss those used to eliminate EOF, to tune EOF, to prevent analyte adsorption, or to perform multiple functions. The knowledgeable application of the most promising recent developments in this area will allow for the separation of complex protein mixtures and for the development of novel microchannel wall modifications.
在基于毛细管和芯片的生物分子电泳分析中，微通道壁涂层的必要性已得到充分理解。对电渗流（EOF）的调节或消除以及对分析物吸附的防止，对于在微通道内快速、高效地分离蛋白质和DNA至关重要。微通道壁涂层和其他壁修饰对于蛋白质分离尤为关键，无论是在熔融石英毛细管中，还是在玻璃或聚合物微流控装置中。在本综述中，我们讨论了三大类微通道壁涂层的最新进展——共价连接的聚合物涂层、物理吸附的聚合物涂层和小分子添加剂。我们还简要回顾了对聚合物微流控装置有用的修饰。在每一类壁涂层中，我们讨论了那些用于消除EOF、调节EOF、防止分析物吸附或执行多种功能的涂层。对该领域最有前景的最新进展的明智应用将有助于分离复杂的蛋白质混合物，并开发新型的微通道壁修饰。
