Department of Chemistry, University of Kansas, Lawrence, Kansas, USA.
J Sep Sci. 2021 Feb;44(3):744-751. doi: 10.1002/jssc.202000883. Epub 2020 Dec 13.
Separation of a set of model proteins was tested on a microchip electrophoresis analytical platform capable of sample injection by two different electrokinetic mechanisms. A range of separation modes-microchip capillary zone electrophoresis, microchip micellar electrokinetic chromatography, and nanoparticle-based sieving-was tested on glass and polydimethylsiloxane/glass microchips and with silica-nanoparticle colloidal arrays. The model proteins calmodulin (18 kiloDalton), bovine serum albumin (66 kDa), and concanavalin (106 kDa) were labeled with Alexa Fluor 647 for laser-induced fluorescence detection. The best separation and resolution were obtained in a silica-nanoparticle colloidal array chip.
采用能够通过两种不同电动机制进行样品注入的微芯片电泳分析平台，对一组模型蛋白质进行了分离测试。在玻璃和聚二甲基硅氧烷/玻璃微芯片上，采用了一系列分离模式-微芯片毛细管区带电泳、微芯片胶束电动色谱和基于纳米颗粒的筛分-并对其进行了测试，同时还使用了基于纳米颗粒的胶体阵列。用 Alexa Fluor 647 对钙调蛋白（18 千道尔顿）、牛血清白蛋白（66 kDa）和伴刀豆球蛋白（106 kDa）等模型蛋白质进行了标记，以便激光诱导荧光检测。在基于纳米颗粒的胶体阵列芯片上获得了最佳的分离和分辨率。
