Department of Chemistry, University of Michigan, 930 N. University Ave, Ann Arbor, Michigan 48109, USA.
Analyst. 2017 May 30;142(11):1847-1866. doi: 10.1039/c7an00198c.
This review article describes the significant recent advances in the analysis of proteins by capillary and microchip electrophoresis during the period from mid-2014 to early 2017. This review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. The protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.
这篇综述文章描述了 2014 年年中至 2017 年初期间毛细管电泳和微芯片电泳分析蛋白质的重要最新进展。本文重点介绍了在人体标本、动物组织和植物样本中进行高效蛋白质分析的进展、新方法、仪器创新改造以及面临的挑战。本文涵盖的蛋白质分析领域包括天然、还原和变性蛋白质的分析,以及 Western 印迹、蛋白质治疗和蛋白质组学。