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毛细管电泳和微芯片电泳在蛋白质分析中的最新进展。

Recent advances in protein analysis by capillary and microchip electrophoresis.

机构信息

Department of Chemistry, University of Michigan, 930 N. University Ave, Ann Arbor, Michigan 48109, USA.

出版信息

Analyst. 2017 May 30;142(11):1847-1866. doi: 10.1039/c7an00198c.

DOI:10.1039/c7an00198c
PMID:28470231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5516626/
Abstract

This review article describes the significant recent advances in the analysis of proteins by capillary and microchip electrophoresis during the period from mid-2014 to early 2017. This review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. The protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.

摘要

这篇综述文章描述了 2014 年年中至 2017 年初期间毛细管电泳和微芯片电泳分析蛋白质的重要最新进展。本文重点介绍了在人体标本、动物组织和植物样本中进行高效蛋白质分析的进展、新方法、仪器创新改造以及面临的挑战。本文涵盖的蛋白质分析领域包括天然、还原和变性蛋白质的分析,以及 Western 印迹、蛋白质治疗和蛋白质组学。

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本文引用的文献

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Do the complementarities of electrokinetic and chromatographic procedures represent the "Swiss knife" in proteomic investigation? An overview of the literature in the past decade.电动和色谱方法的互补性在蛋白质组学研究中是否代表了“瑞士军刀”?过去十年文献综述。
Electrophoresis. 2017 Jun;38(12):1538-1550. doi: 10.1002/elps.201600504. Epub 2017 Feb 21.
3
Development of a multichannel microfluidic system with Schlieren imaging microscopy for online chip-based moving boundary electrophoresis.用于在线芯片式移动边界电泳的具有纹影成像显微镜的多通道微流控系统的开发。
J Chromatogr A. 2017 Feb 10;1484:93-97. doi: 10.1016/j.chroma.2017.01.007. Epub 2017 Jan 3.
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Parallel analysis and orthogonal identification of N-glycans with different capillary electrophoresis mechanisms.采用不同毛细管电泳机制对 N-聚糖进行平行分析和正交鉴定。
Anal Chim Acta. 2017 Feb 8;953:79-86. doi: 10.1016/j.aca.2016.11.043. Epub 2016 Nov 25.
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Evaluation of a commercial electro-kinetically pumped sheath-flow nanospray interface coupled to an automated capillary zone electrophoresis system.对一种与自动毛细管区带电泳系统联用的商用电动泵鞘流纳米喷雾接口的评估。
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