Zhou Jun F, Cai Dong, Tong Gui Z
Second Affiliated Hospital, College of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejiang Province, People's Republic of China.
Free Radic Res. 2003 Feb;37(2):137-43. doi: 10.1080/1071576021000036623.
To study the relationship between oxidative stress and potential free radical damage associated with photocopying and to explore a role for ozone emitted during the photocopying process.
80 photocopying operators (PO) and 80 healthy volunteers (HV) were enrolled in a random control study design, in which the level of lipoperoxide (LPO, thiobarbituric acid reactive substances, TBARS) in erythrocytes and the levels of vitamin C (VC), vitamin E (VE) and beta-carotene (beta-CAR) in plasma as well as the activities of superoxide dismutase (SOD) and catalase (CAT) in erythrocytes were determined by spectrophotometric methods.
Compared with the average values of the above biochemical parameters in the HV group, the average value of LPO (TBARS) in erythrocytes in the PO group was significantly increased (P < 0.0001), while the average values of VC, VE and beta-CAR in plasma as well as those of SOD and CAT in erythrocytes in the PO group were significantly decreased (P < 0.0001). Pearson product-moment correlation analysis showed that with the increase of the ozone level in photocopying sites and the PO duration of exposure to ozone, the level of LPO in erythrocytes in the operators was increased (P < 0.001), while the levels of VC, VE and beta-CAR in plasma as well as the activities of SOD and CAT in erythrocytes in the operators were decreased (P < 0.01-0.0001).
The findings in this study suggest that ozone causes oxidative damage in copier operatives.
研究与复印相关的氧化应激和潜在自由基损伤之间的关系，并探讨复印过程中释放的臭氧所起的作用。
采用随机对照研究设计，纳入80名复印操作员（PO）和80名健康志愿者（HV），通过分光光度法测定红细胞中脂过氧化物（LPO，硫代巴比妥酸反应性物质，TBARS）水平、血浆中维生素C（VC）、维生素E（VE）和β-胡萝卜素（β-CAR）水平以及红细胞中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。
与HV组上述生化参数的平均值相比，PO组红细胞中LPO（TBARS）的平均值显著升高（P < 0.0001），而PO组血浆中VC、VE和β-CAR的平均值以及红细胞中SOD和CAT的平均值显著降低（P < 0.0001）。Pearson积矩相关分析表明，随着复印场所臭氧水平的升高以及操作员接触臭氧的时间延长，操作员红细胞中LPO水平升高（P < 0.001），而血浆中VC、VE和β-CAR水平以及红细胞中SOD和CAT活性降低（P < 0.01 - 0.0001）。
本研究结果表明，臭氧会对复印机操作人员造成氧化损伤。
