Ulus A T, Aksoyek A, Ozkan M, Katircioglu S F, Basu S
Cardiovascular Surgery Clinic, Ozel Yasam Hospital, Ankara, Turkey.
Free Radic Biol Med. 2003 Apr 1;34(7):911-7. doi: 10.1016/s0891-5849(03)00030-3.
Free radicals are believed to be involved in postsurgery-related complications. We studied whether cardiopulmonary bypass (CPB) operation has any immediate impact on the initiation of oxidative stress and inflammatory response by measuring isoprostanes and prostaglandin F2alpha during and 24 h following CPB. The levels of 8-iso-PGF2alpha (a major F2-isoprostane and biomarker of oxidative stress) and 15-keto-dihydro-PGF2alpha (a major metabolite of PGF2alpha and biomarker of inflammatory response) were measured in frequently collected plasma samples before, during, and up to 24 h postsurgery in 21 patients. 8-Iso-PGF2alpha levels significantly increased within 3 min (p <.0001) and continued until 50 min (p <.0001) during CPB. On the contrary, no significant increase of inflammatory response indicator, 15-keto-dihydro-PGF2alpha was found during and up to 24 h postoperatively. These findings establish an increased free radical-induced oxidative stress activity rather than inflammatory response after CPB.
自由基被认为与术后相关并发症有关。我们通过在体外循环（CPB）期间及CPB后24小时测量异前列腺素和前列腺素F2α，研究了CPB手术是否对氧化应激和炎症反应的启动有任何直接影响。在21例患者手术前、手术期间以及术后长达24小时的频繁采集的血浆样本中，测量了8-异前列腺素F2α（一种主要的F2-异前列腺素和氧化应激生物标志物）和15-酮-二氢-前列腺素F2α（前列腺素F2α的主要代谢产物和炎症反应生物标志物）的水平。在CPB期间，8-异前列腺素F2α水平在3分钟内显著升高（p<.0001），并持续至50分钟（p<.0001）。相反，在术后及术后长达24小时内，未发现炎症反应指标15-酮-二氢-前列腺素F2α有显著升高。这些发现表明，CPB后自由基诱导的氧化应激活性增加，而非炎症反应增加。
