基于几丁质合成酶1（CHS1）基因序列直接检测皮肤样本中的皮肤癣菌。

Direct detection of dermatophytes in skin samples based on sequences of the chitin synthase 1 (CHS1) gene.

作者信息

Kano Rui, Hirai Asuka, Muramatsu Masato, Watari Toshihiro, Hasegawa Atsuhiko

机构信息

Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa, Japan.

出版信息

J Vet Med Sci. 2003 Feb;65(2):267-70. doi: 10.1292/jvms.65.267.

DOI:10.1292/jvms.65.267

PMID:12655126

Abstract

For the direct detection of dermatophytes in skin scrapings and hairs from animals, a primer pair specific to the chitin synthase 1 (CHS1) gene of dermatophytes was constructed. By PCR analysis with the primer pair, dermatophyte DNA could be diagnosed directly and rapidly in clinical skin samples.

摘要

为了直接检测动物皮肤刮屑和毛发中的皮肤癣菌，构建了一对针对皮肤癣菌几丁质合酶1（CHS1）基因的特异性引物。通过使用该引物对进行PCR分析，可在临床皮肤样本中直接快速诊断皮肤癣菌DNA。