Garg Jaya, Tilak Ragini, Singh Sanjay, Gulati Anil Kumar, Garg Atul, Prakash Pradyot, Nath Gopal
Department of Microbiology, Banaras Hindu University, Varanasi, India.
J Clin Microbiol. 2007 Oct;45(10):3443-5. doi: 10.1128/JCM.02367-06. Epub 2007 Aug 15.
In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.
在本研究中，针对152例临床疑似甲真菌病患者，将使用靶向几丁质合成酶1基因（CHS1）泛皮肤癣菌特异性序列的新型引物进行的巢式PCR与氢氧化钾显微镜检查、培养分离及单轮PCR用于诊断的情况进行了比较。结果表明，对于病因是皮肤癣菌的甲真菌病病例，巢式PCR可被视为诊断的金标准。
