泛皮肤癣菌巢式PCR在甲癣诊断中的评估
Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis.
作者信息
Garg Jaya, Tilak Ragini, Singh Sanjay, Gulati Anil Kumar, Garg Atul, Prakash Pradyot, Nath Gopal
机构信息
Department of Microbiology, Banaras Hindu University, Varanasi, India.
出版信息
J Clin Microbiol. 2007 Oct;45(10):3443-5. doi: 10.1128/JCM.02367-06. Epub 2007 Aug 15.
Abstract
In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.
摘要
在本研究中，针对152例临床疑似甲真菌病患者，将使用靶向几丁质合成酶1基因（CHS1）泛皮肤癣菌特异性序列的新型引物进行的巢式PCR与氢氧化钾显微镜检查、培养分离及单轮PCR用于诊断的情况进行了比较。结果表明，对于病因是皮肤癣菌的甲真菌病病例，巢式PCR可被视为诊断的金标准。
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本文引用的文献
1
Identification of Trichophyton rubrum by nested PCR analysis from paraffin embedded specimen in trichophytia profunda acuta of the glabrous skin.通过巢式PCR分析从无毛皮肤急性深部癣病石蜡包埋标本中鉴定红色毛癣菌。
Nihon Ishinkin Gakkai Zasshi. 2005;46(2):129-32. doi: 10.3314/jjmm.46.129.
2
Polymerase chain reaction in the diagnosis of onychomycosis.聚合酶链反应在甲癣诊断中的应用
Eur J Dermatol. 2004 Jan-Feb;14(1):52-5.
3
Direct detection of dermatophytes in skin samples based on sequences of the chitin synthase 1 (CHS1) gene.基于几丁质合成酶1（CHS1）基因序列直接检测皮肤样本中的皮肤癣菌。
J Vet Med Sci. 2003 Feb;65(2):267-70. doi: 10.1292/jvms.65.267.
4
Facing up to the diagnostic uncertainty and management of onychomycoses.直面甲癣的诊断不确定性与治疗
Int J Dermatol. 1999 Sep;38 Suppl 2:1-6. doi: 10.1046/j.1365-4362.1999.00007.x.
5
Onychomycosis: therapeutic update.
J Am Acad Dermatol. 1999 Jun;40(6 Pt 2):S21-6. doi: 10.1016/s0190-9622(99)70397-x.
6
Detection and differentiation of causative fungi of onychomycosis using PCR amplification and restriction enzyme analysis.利用聚合酶链反应扩增和限制性内切酶分析检测及鉴别甲癣的致病真菌
Int J Dermatol. 1998 Sep;37(9):682-6. doi: 10.1046/j.1365-4362.1998.00517.x.
7
Onychomycosis: pathogenesis, diagnosis, and management.甲癣：发病机制、诊断与治疗
Clin Microbiol Rev. 1998 Jul;11(3):415-29. doi: 10.1128/CMR.11.3.415.
8
Fungal nail disease: a guide to good practice (report of a Working Group of the British Society for Medical Mycology).甲真菌病：良好实践指南（英国医学真菌学会工作组报告）
BMJ. 1995 Nov 11;311(7015):1277-81. doi: 10.1136/bmj.311.7015.1277.
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