一种用于鉴定宠物犬小孢子菌和须癣毛癣菌感染的一步法聚合酶链反应（PCR）方法的应用。

The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets.

作者信息

Dąbrowska Iwona, Dworecka-Kaszak Bożena, Brillowska-Dąbrowska Anna

机构信息

Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warszawa, Poland.

Gdansk University of Technology, Department of Microbiology, Gdańsk, Poland.

出版信息

Acta Biochim Pol. 2014;61(2):375-8. Epub 2014 Jun 18.

PMID:24945136

Abstract

INTRODUCTION

Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin.

THE AIM

Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming.

MATERIALS AND METHODS

In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska-Dąbrowska A, Saunte DM, Arenderup MC, 2007, Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200-1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes.

RESULTS

The method used here is able to confirm the presence of dermatophyte DNA in pure cultures in less than 5 hours.

摘要

引言

皮肤癣菌是一组亲缘关系密切的嗜角质真菌。它们是浅表真菌感染的重要病原体。这些真菌能够侵入人和动物的角质化组织，引起毛发、指甲或皮肤的皮肤癣菌病（癣）。

目的

传统的癣诊断基于培养真菌的形态学鉴定，耗时较长。

材料与方法

在本研究中，我们应用了由布里洛娃-达布罗夫斯卡及其同事获得专利的一种方法（布里洛娃-达布罗夫斯卡A、桑特DM、阿伦德鲁普MC，2007年，用特异性检测红色毛癣菌的方法在5小时内诊断皮肤癣菌指甲感染。《临床微生物学杂志》45：1200-1204），该方法包括提取真菌DNA并用泛皮肤癣菌引物进行PCR扩增，以确认皮肤癣菌的存在。