普马拉汉坦病毒在丹麦的分布：对来自菲英岛和日德兰半岛的棕背䶄（黄毛鼠耳蝠）的分析。

Distribution of Puumala hantavirus in Denmark: analysis of bank voles (Clethrionomys glareolus) from Fyn and Jutland.

作者信息

Sironen Tarja, Plyusnina Angelina, Andersen Hans Kerzel, Lodal Jens, Leirs Herwig, Niemimaa Jukka, Henttonen Heikkia, Vaheri Antti, Lundkvist Ake, Plyusnin Alexander

机构信息

Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland.

出版信息

Vector Borne Zoonotic Dis. 2002 Spring;2(1):37-45. doi: 10.1089/153036602760260760.

DOI:10.1089/153036602760260760

PMID:12656129

Abstract

The majority of hantaviral infections in Denmark since 1957 have occurred on the island of Fyn. We have recently shown the presence of Puumala hantavirus (PUUV) in bank voles (Clethrionomys glareolus) on Fyn. To learn more about the distribution and prevalence of PUUV in Denmark, 310 small mammals, belonging to six rodent and two insectivore species, were trapped in four localities on Fyn and three localities on the Jutland Peninsula (mainland Denmark), where only a few cases of nephropathia epidemica (NE) have been confirmed so far. Serum samples (heart extracts) from all animals were initially analyzed for the presence of hantavirus-specific antibodies (Ab) by enzyme immunoassay, and lung tissue samples of bank voles for the presence of the viral nucleocapsid protein antigen by immunoblotting. Altogether 11 Ab-positive rodents were found, all of them bank voles from Fyn. PUUV genome sequences (nucleotides 2,168-2,569 from the medium genomic segment) were recovered by reverse transcription-polymerase chain reaction from seven bank voles. They showed an overall diversity up to 5% and formed a well-supported genetic lineage on the phylogenetic tree with a clustering of two strains from southeastern Fyn together and a separation from the one strain from western Fyn. Our data confirmed the circulation of PUUV in both the southeastern and western parts of the island, known to have the highest numbers of human NE cases.

摘要

自1957年以来，丹麦的大多数汉坦病毒感染发生在菲英岛。我们最近发现菲英岛的棕背鼠（黄毛姬鼠）体内存在普马拉汉坦病毒（PUUV）。为了进一步了解PUUV在丹麦的分布和流行情况，我们在菲英岛的四个地点以及日德兰半岛（丹麦本土）的三个地点捕获了310只小型哺乳动物，这些动物分属于6种啮齿动物和2种食虫动物，日德兰半岛目前仅确诊了少数几例流行性肾病（NE）病例。首先通过酶免疫测定分析所有动物的血清样本（心脏提取物）中是否存在汉坦病毒特异性抗体（Ab），并通过免疫印迹法分析棕背鼠的肺组织样本中是否存在病毒核衣壳蛋白抗原。总共发现了11只抗体呈阳性的啮齿动物，均为来自菲英岛的棕背鼠。通过逆转录聚合酶链反应从7只棕背鼠中获得了PUUV基因组序列（中基因组片段的核苷酸2168 - 2569）。它们的总体差异高达5%，在系统发育树上形成了一个得到充分支持的遗传谱系，其中来自菲英岛东南部的两个毒株聚在一起，与来自菲英岛西部的一个毒株分离。我们的数据证实了PUUV在该岛东南部和西部的传播，这两个地区的人类NE病例数最多。