比利时普马拉汉坦病毒株的基因特征：一个独特系统发育谱系的证据
Genetic characterization of Puumala hantavirus strains from Belgium: evidence for a distinct phylogenetic lineage.
作者信息
Escutenaire S, Chalon P, Heyman P, Van der Auwera G, van der Groen G, Verhagen R, Thomas I, Karelle-Bui L, Vaheri A, Pastoret P P, Plyusnin A
机构信息
Department of Immunology-Vaccinology, Faculty of Veterinary Medicine, University of Liège, Bât B 43 bis, Boulevard de Colonster, 20. B-4000, Liège, Belgium.
出版信息
Virus Res. 2001 Apr;74(1-2):1-15. doi: 10.1016/s0168-1702(00)00224-0.
Puumala hantavirus (PUUV) sequences were recovered from red bank voles (Clethrionomys glareolus) trapped between 1996 and 1998 in four localities of southern Belgium: Thuin, Montbliart, Momignies and Couvin. In addition, three PUUV isolates originating from bank voles trapped in the 1980s in southern (Montbliart) and northern (Turnhout) Belgium were genetically characterized. Analysis of the complete S and partial M segment sequences showed that the Belgian PUUV strains constitute a genetic lineage, distinct from other known PUUV lineages from Europe and Japan. This lineage also includes a wild strain (Cg-Erft) originating from a neighbouring area of Germany. Within the Belgian lineage, geographical clustering of genetic variants was observed. In the Montbliart site, the range of diversity between the most temporally distant strains (from 1986 and 1996-1998) was higher than between those from 1996 and 1998, suggesting slight genetic drift via accumulation of neutral or quasi-neutral substitutions with time.
1996年至1998年期间，在比利时南部的图安、蒙布里阿尔、莫米尼和库万四个地区捕获的红背田鼠（棕背䶄）体内分离出普马拉汉坦病毒（PUUV）序列。此外，对20世纪80年代在比利时南部（蒙布里阿尔）和北部（特尔讷豪特）捕获的田鼠中分离出的三株PUUV进行了基因特征分析。对完整的S片段和部分M片段序列的分析表明，比利时的PUUV毒株构成了一个遗传谱系，与欧洲和日本的其他已知PUUV谱系不同。这个谱系还包括一株源自德国邻近地区的野生毒株（Cg-Erft）。在比利时谱系内，观察到了遗传变异的地理聚类现象。在蒙布里阿尔地区，时间上距离最远的毒株（来自1986年和1996 - 1998年）之间的多样性范围高于1996年和1998年的毒株之间的多样性，这表明随着时间的推移，通过中性或准中性替代的积累发生了轻微的遗传漂变。
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