Alazard D, Dukan S, Urios A, Verhé F, Bouabida N, Morel F, Thomas P, Garcia J-L, Ollivier B
IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
Laboratoire de Microbiologie Marine, CNRS-INSU-UMR 6117, Université de la Méditerranée, Marseille Luminy, France.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):173-178. doi: 10.1099/ijs.0.02323-0.
Mesophilic, hydrogenotrophic, sulfate-reducing bacteria were isolated from a deep-sea hydrothermal chimney sample collected at 13 degrees N on the East-Pacific Rise at a depth of 2,600 m. Two strains (BL5 and H9) were found to be phylogenetically similar to Desulfovibrio profundus (similarity >99%), whereas two other strains (H1 and AM13T) were found to be phylogenetically distinct (similarity 96.4%) from Desulfovibrio zosterae, their closest relative. Strain AM13T was characterized further. It was a barophilic, Gram-negative, non-sporulating, motile, vibrio-shaped or sigmoid bacterium possessing desulfoviridin. It grew at temperatures ranging from 20 to 40 degrees C, with an optimum at 35 degrees C in the presence of 2.5% NaCl. The pH range for growth was 6.7-8.2 with an optimum around 7.8. Strain AM13T utilized H2/CO2, lactate, formate, ethanol, choline and glycerol as electron donors. Electron acceptors were sulfate, sulfite and thiosulfate, but not elemental sulfur or nitrate. The G + C content of DNA was 47 mol%. Strain AM13T (= DSM 14728T = CIP107303T) differed from D. zosterae not only phylogenetically, but also genomically (DNA-DNA reassociation value between the two bacteria was 23.8%) and phenotypically. This isolate is therefore proposed as the type strain of a novel species of the genus Desulfovibrio, Desulfovibrio hydrothermalis sp. nov.
嗜温、氢营养型、硫酸盐还原菌是从东太平洋海隆北纬13度、深度2600米处采集的深海热液烟囱样本中分离得到的。发现两株菌株（BL5和H9）在系统发育上与深海脱硫弧菌相似（相似度>99%），而另外两株菌株（H1和AM13T）在系统发育上与它们亲缘关系最近的海草脱硫弧菌明显不同（相似度96.4%）。对菌株AM13T进行了进一步表征。它是一种嗜压、革兰氏阴性、不产芽孢、具运动性、弧状或S形的细菌，含有脱硫绿素。它在20至40摄氏度的温度范围内生长，在2.5%氯化钠存在的情况下，最适生长温度为35摄氏度。生长的pH范围是6.7 - 8.2，最适pH约为7.8。菌株AM13T利用H2/CO2、乳酸、甲酸、乙醇、胆碱和甘油作为电子供体。电子受体是硫酸盐、亚硫酸盐和硫代硫酸盐，但不是元素硫或硝酸盐。DNA的G + C含量为47摩尔%。菌株AM13T（= DSM 14728T = CIP107303T）不仅在系统发育上与海草脱硫弧菌不同，在基因组上（两种细菌之间的DNA - DNA重结合值为23.8%）和表型上也不同。因此，该分离株被提议作为脱硫弧菌属一个新物种——热液脱硫弧菌的模式菌株。
