Doronina Nina V, Darmaeva Tsyregma D, Trotsenko Yuri A
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow region 142290, Russia.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):223-229. doi: 10.1099/ijs.0.02267-0.
A moderately haloalkaliphilic and obligately methylotrophic bacterium (strain M39T) with the ribulose monophosphate pathway of carbon assimilation is described. Cells of this methanol and methylamine utilizer are aerobic, Gram-negative, asporogenous, motile short rods, multiplying by binary fission. It is auxotrophic for vitamin B12 and requires NaHCO3 or NaCl for growth in alkaline medium. Its cellular fatty acid profile consists primarily of straight-chain saturated C16:0 and unsaturated C16:1 and C18:1 acids. The major ubiquinone is Q-8. The dominant phospholipids are phosphatidylethanolamine and phosphatidylglycerol. Diphosphatidylglycerol is also present. Optimal growth conditions are 25-29 degrees C, pH 9.0-9.5 and 3-4 % (w/v) NaCl. Cells accumulate the cyclic amino acid ectoine as the main compatible solute. The DNA G + C content is 48.3 mol%. Based on 16S rDNA sequence analysis and DNA-DNA relatedness (25-30 %) with the type strains of marine methylotrophs belonging to the genus Methylophaga, the novel isolate M39T (=VKM B-2251T =ATCC BAA-297T) was classified as the type strain of Methylophaga alcalica sp. nov.
描述了一种具有核糖单磷酸碳同化途径的中度嗜盐碱兼性甲基营养型细菌（菌株M39T）。这种利用甲醇和甲胺的细菌细胞为需氧型、革兰氏阴性、无芽孢、运动性短杆菌，通过二分分裂繁殖。它对维生素B12营养缺陷，在碱性培养基中生长需要NaHCO3或NaCl。其细胞脂肪酸谱主要由直链饱和C16:0以及不饱和C16:1和C18:1酸组成。主要泛醌为Q-8。主要磷脂为磷脂酰乙醇胺和磷脂酰甘油。也存在双磷脂酰甘油。最佳生长条件为25-29℃、pH 9.0-9.5和3-4%（w/v）NaCl。细胞积累环状氨基酸羟基四氢嘧啶作为主要相容性溶质。DNA的G + C含量为48.3 mol%。基于16S rDNA序列分析以及与属于噬甲基菌属的海洋甲基营养型模式菌株的DNA-DNA相关性（25-30%），新分离株M39T（=VKM B-2251T =ATCC BAA-297T）被分类为嗜碱噬甲基菌（Methylophaga alcalica）新种的模式菌株。
