Microbial Culture Collection, National Centre for Cell Science, Pune 411007, India.
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow 142290, Russia.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1613-1618. doi: 10.1099/ijs.0.035089-0. Epub 2011 Sep 2.
A moderately haloalkaliphilic methylotrophic bacterium possessing the ribulose monophosphate pathway for carbon assimilation, designated MPL(T), was isolated from Lonar Lake sediment microcosms that were oxidizing methane for two weeks. The isolate utilized methanol and was an aerobic, Gram-negative, asporogenous, motile, short rod that multiplied by binary fission. The isolate required NaHCO(3) or NaCl for growth and, although not auxotrophic for vitamin B(12), had enhanced growth with vitamin B(12). Optimal growth occurred with 0.5-2% (w/v) NaCl, at 28-30 °C and at pH 9.0-10.0. The cellular fatty acid profile consisted primarily of straight-chain saturated C(16:0) and unsaturated C(16:1)ω7c and C(18:1)ω7c. The major ubiquinone was Q-8. The dominant phospholipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Cells accumulated ectoine as the main compatible solute. The DNA G+C content was 50.0 mol%. The isolate exhibited 94.0-95.4% 16S rRNA gene sequence similarity with the type strains of methylotrophs belonging to the genus Methylophaga and 31% DNA-DNA relatedness with the reference strain, Methylophaga alcalica VKM B-2251(T). It is proposed that strain MPL(T) represents a novel species, Methylophaga lonarensis sp. nov. (type strain MPL(T)=VKM B-2684(T)=MCC 1002(T)).
一株中度嗜盐嗜碱的甲基营养型细菌，具有核酮糖单磷酸途径进行碳同化，命名为 MPL(T)，从 Lonar 湖沉积物微宇宙中分离出来，这些微宇宙已经将甲烷氧化了两周。该分离株利用甲醇，是一种需氧、革兰氏阴性、无孢子、能动、短杆状的细菌，通过二分分裂繁殖。该分离株的生长需要 NaHCO(3)或 NaCl，虽然不需要维生素 B(12)，但添加维生素 B(12)可以增强生长。最佳生长条件为 0.5-2%（w/v）NaCl、28-30°C 和 pH9.0-10.0。细胞脂肪酸组成主要由直链饱和 C(16:0)和不饱和 C(16:1)ω7c 和 C(18:1)ω7c 组成。主要的类质体醌是 Q-8。主要的磷脂是磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。细胞积累海藻糖作为主要的相容性溶质。DNA G+C 含量为 50.0 mol%。该分离株与属于甲基营养菌属的甲基营养型菌株的 16S rRNA 基因序列相似性为 94.0-95.4%，与参考菌株 Methylophaga alcalica VKM B-2251(T)的 DNA-DNA 相关性为 31%。建议该菌株 MPL(T)代表一个新种，即 Lonarensis sp. nov.甲基营养菌（模式株 MPL(T)=VKM B-2684(T)=MCC 1002(T)）。
