Petrosyan Pavel, García-Varela Martín, Luz-Madrigal Agustín, Huitrón Carlos, Flores María Elena
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, AP 70228, UNAM, 04510, México DF, Mexico.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):269-273. doi: 10.1099/ijs.0.02251-0.
The taxonomic position of a thermophilic actinomycete strain isolated from soil was examined using a polyphasic approach. The strain, designated CH-M-1035T, was assigned to the genus Streptomyces on the basis of chemical and morphological criteria. It formed Rectiflexibiles aerial hyphae that carried long chains of rounded, smooth spores. The almost complete nucleotide sequence of the 16S rRNA gene of strain CH-M-1035T was determined and its comparison with the 16S rDNA sequences of previously studied streptomycetes confirmed the assignment of the novel strain to the genus Streptomyces. Strain CH-M-1035T clustered with species belonging to the Streptomyces thermodiastaticus clade in the 1 6S-rDNA-based phylogenetic tree. However, the phenotypic properties of strain CH-M-1035T differed from those of the recognized species within this clade. Therefore, it is proposed that strain CH-M-1035T be classified as a novel species within the genus Streptomyces, as Streptomyces mexicanus (type strain CH-M-1035T =DSM 41796T =BM-B-384T =NRRL B-24196T).
采用多相分类法对从土壤中分离得到的嗜热放线菌菌株的分类地位进行了研究。该菌株命名为CH-M-1035T,根据化学和形态学标准被归入链霉菌属。它形成了直形弯曲的气生菌丝,带有长链状圆形、光滑的孢子。测定了菌株CH-M-1035T的16S rRNA基因的几乎完整的核苷酸序列,并将其与先前研究的链霉菌的16S rDNA序列进行比较,证实了该新菌株属于链霉菌属。在基于16S-rDNA的系统发育树中,菌株CH-M-1035T与属于嗜热淀粉酶链霉菌分支的物种聚类在一起。然而,菌株CH-M-1035T的表型特征与该分支内已确认的物种不同。因此,建议将菌株CH-M-1035T分类为链霉菌属内的一个新物种,命名为墨西哥链霉菌(模式菌株CH-M-1035T =DSM 41796T =BM-B-384T =NRRL B-24196T)。