Ping Xu, Takahashi Yōko, Seino Akio, Iwai Yuzuru, Ōmura Satoshi
Research Center for Biological Function, The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):577-581. doi: 10.1099/ijs.0.02692-0.
The taxonomic position was determined for a soil actinomycete, isolate KM-4927(T), that produced the antibiotic hitachimycin. The strain was assigned to the genus Streptomyces on the basis of 16S rDNA analysis, where it formed a separate clade. The strain is characterized by grey aerial cell mass, spiral spore chains and a rugose spore surface, menaquinones of the MK-9(H(4), H(2), H(6)) types and cell-wall chemotype I. DNA-DNA reassociation with 21 phylogenetically neighbouring Streptomyces type strains showing similar morphological characteristics to strain KM-4927(T) indicated that this isolate is only moderately related to other Streptomyces species. On the basis of genomic and physiological properties, the novel species Streptomyces scabrisporus sp. nov. is proposed; the type strain is strain KM-4927(T) (=JCM 11712(T)=NRRL B-24202(T)).
确定了产生抗生素北里霉素的土壤放线菌菌株KM-4927(T)的分类地位。基于16S rDNA分析,该菌株被归入链霉菌属,在该属中它形成一个独立的分支。该菌株的特征为灰色气生菌丝体、螺旋状孢子链和具皱纹的孢子表面,MK-9(H(4), H(2), H(6))类型的甲基萘醌以及细胞壁化学类型I。与21株在系统发育上相邻且形态特征与菌株KM-4927(T)相似的链霉菌模式菌株进行DNA-DNA重联分析表明,该分离株与其他链霉菌物种的亲缘关系仅为中度相关。基于基因组和生理特性,提出了新种粗糙孢子链霉菌(Streptomyces scabrisporus sp. nov.);模式菌株为菌株KM-4927(T) (=JCM 11712(T)=NRRL B-24202(T))。