Laboratory of Microbiology, Department of Botany, Christ Church College , Kanpur, 208001 , India.
Braz J Microbiol. 2012 Oct;43(4):1545-52. doi: 10.1590/S1517-838220120004000039. Epub 2012 Jun 1.
Agro-industrial wastes such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw are cheapest and abundantly available natural carbon sources. The present study was aimed to production of amylase and xylanase simultaneously using agro-industrial waste as the sole carbon source. Seven thermophilic strains of actinomycete were isolated from the mushroom compost. Among of these, strain designated MSC702 having high potential to utilize agro-industrial wastes for the production of amylase and xylanase. Strain MSC702 was identified as novel species of Streptomyces through morphological characterization and 16S rRNA gene sequence. Enzyme production was determined using 1% (w/v) of various agro-industrial waste in production medium containing (g/100mL): K2HPO4 (0.1), (NH4)2SO4 (0.1), NaCl (0.1), MgSO4 (0.1) at pH 7.0 after incubation of 48 h at 50°C. The amylase activity (373.89 IU/mL) and xylanase activity (30.15 IU/mL) was maximum in rice bran. The decreasing order of amylase and xylanase activity in different type of agro-industrial wastes were found rice bran (RB) > corn cob (CC) > wheat bran (WB) > wheat straw (WS) > sugarcane bagasse (SB) and rice bran (RB) > wheat bran (WB) > wheat straw (WS) > sugarcane bagasse (SB) > corn cob (CC), respectively. Mixed effect of different agro-industrial wastes was examined in different ratios. Enzyme yield of amylase and xylanase was ~1.3 and ~2.0 fold higher with RB: WB in 1:2 ratio.
农业工业废物如甘蔗渣、麦麸、米糠、玉米芯和麦秆是最便宜和最丰富的天然碳源。本研究旨在利用农业工业废物作为唯一碳源同时生产淀粉酶和木聚糖酶。从蘑菇堆肥中分离出 7 株嗜热放线菌。其中，菌株 MSC702 具有利用农业工业废物生产淀粉酶和木聚糖酶的高潜力。通过形态特征和 16S rRNA 基因序列鉴定，菌株 MSC702 被鉴定为新型链霉菌。在含有（g/100mL）：K2HPO4（0.1）、（NH4）2SO4（0.1）、NaCl（0.1）、MgSO4（0.1）的生产培养基中，用 1%（w/v）的各种农业工业废物进行酶生产，在 50°C 下孵育 48 小时后，pH 值为 7.0。在米糠中，淀粉酶活性（373.89IU/mL）和木聚糖酶活性（30.15IU/mL）最高。在不同类型的农业工业废物中，淀粉酶和木聚糖酶活性的降低顺序为米糠（RB）>玉米芯（CC）>麦麸（WB）>麦秆（WS）>甘蔗渣（SB）和米糠（RB）>麦麸（WB）>麦秆（WS）>甘蔗渣（SB）>玉米芯（CC）。在不同比例下研究了不同农业工业废物的混合效应。在 RB:WB 为 1:2 的比例下，淀粉酶和木聚糖酶的酶产量分别提高了1.3 倍和2.0 倍。
