Chun J, Youn H D, Yim Y I, Lee H, Kim M Y, Hah Y C, Kang S O
Department of Microbiology, College of Natural Sciences, Seoul National University, Republic of Korea.
Int J Syst Bacteriol. 1997 Apr;47(2):492-8. doi: 10.1099/00207713-47-2-492.
The taxonomic position of an actinomycete strain isolated from Korean soil was examined by a polyphasic approach. The isolate, designated IMSNU-1, was clearly assigned to the genus Streptomyces on the basis of morphological and chemotaxonomic data. The test strain was the subject of a probabilistic identification study using the identification matrices generated by Langham et al. (J. Gen. Microbiol. 135:121-133, 1989) and found to be marginally close to clusters 19 and 39. An almost complete 16S rRNA gene (rDNA) sequence was obtained for the test strain and compared with those of representative streptomycetes. 16S rDNA sequence data not only support the strain's membership in the genus Streptomyces but also provide strong evidence that our isolate is genealogically distant from representatives of clusters 19 and 39, forming a separate phyletic line in a clade encompassed by streptomycetes. It is therefore proposed from the polyphasic evidence that strain IMSNU-1 be classified in the genus Streptomyces as Streptomyces seoulensis sp. nov.
采用多相分类法对从韩国土壤中分离出的一株放线菌菌株的分类地位进行了研究。该分离株命名为IMSN - 1,根据形态学和化学分类学数据,明确归属于链霉菌属。使用Langham等人(《普通微生物学杂志》135:121 - 133, 1989)生成的鉴定矩阵对测试菌株进行概率鉴定研究,发现其与第19和39簇略有接近。获得了测试菌株几乎完整的16S rRNA基因(rDNA)序列,并与代表性链霉菌的序列进行了比较。16S rDNA序列数据不仅支持该菌株属于链霉菌属,还提供了有力证据,表明我们的分离株在谱系上与第19和39簇的代表菌株相距甚远,在链霉菌涵盖的一个进化枝中形成了一个单独的进化系。因此,根据多相分类证据,建议将菌株IMSN - 1分类为链霉菌属中的首尔链霉菌新种(Streptomyces seoulensis sp. nov.)。