Rosa Adalberto Luiz, Beloti Márcio Mateus
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Braz Dent J. 2003;14(1):16-21. doi: 10.1590/s0103-64402003000100003. Epub 2003 Jul 31.
There is general agreement that rough surfaces improve both biologic and biomechanical responses to titanium (Ti) implants. The aim of this investigation was to study the effect of Ti surface roughness on the response of human bone marrow cell culture evaluating: cell attachment, cell proliferation, total protein content, alkaline phosphatase (ALP) activity, and bone-like nodule formation. Cells were cultured on commercially pure titanium (cpTi) discs with fourdifferent average roughnesses (Ra). For attachment evaluation, cells were cultured for 4 h. After 21 days, cell proliferation, total protein content, and ALP activity were evaluated. For bone-like nodule formation, cells were cultured for 28 days. Data were compared by ANOVA and Duncan's multiple range test. Cell attachment was not affected by surface roughness. For cells cultured on Ti with Ra ranging from 0.80 microm to 1.90 microm, proliferation was reduced while total protein content, and ALP activity were increased. There was a non-statistically significant increase of bone-like nodule formation on a surface with Ra near 0.80 microm. These results suggest that for Ti an Ra ranging from 0.80 microm to 1.90 microm would optimize both intermediary and final cellular responses but not affect the initial response, and a smoother surface would not favor any evaluated response.
人们普遍认为，粗糙表面可改善对钛（Ti）植入物的生物学和生物力学反应。本研究的目的是研究钛表面粗糙度对人骨髓细胞培养反应的影响，评估指标包括：细胞附着、细胞增殖、总蛋白含量、碱性磷酸酶（ALP）活性和骨样结节形成。将细胞接种在具有四种不同平均粗糙度（Ra）的商业纯钛（cpTi）圆盘上。为评估细胞附着情况，细胞培养4小时。培养21天后，评估细胞增殖、总蛋白含量和ALP活性。为观察骨样结节形成情况，细胞培养28天。数据通过方差分析和邓肯多重范围检验进行比较。细胞附着不受表面粗糙度的影响。对于接种在Ra为0.80微米至1.90微米的钛表面上的细胞，增殖减少，而总蛋白含量和ALP活性增加。在Ra接近0.80微米的表面上，骨样结节形成有非统计学意义的增加。这些结果表明，对于钛而言，Ra为0.80微米至1.90微米可优化细胞的中期和最终反应，但不影响初始反应，而更光滑的表面则不利于任何评估的反应。
