Rosa Adalberto L, Beloti Márcio M, van Noort Richard
Department of Oral and Maxillofacial Surgery, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av. do Cafe, s/n, 14040-904, Ribeirao Preto, SP, Brazil.
Dent Mater. 2003 Dec;19(8):768-72. doi: 10.1016/s0109-5641(03)00024-1.
Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute.
The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on the response of the rat bone marrow cells, evaluating: cell attachment, proliferation, total protein content, alkaline phosphatase (ALP) activity, and bone-like nodule formation.
Cells were cultured on HA discs manufactured by a combination of uniaxial powder pressing and different sintering conditions, with different percentage of microporosity (<5%-HA5, 15%-HA15, and 30%-HA30). For attachment evaluation, cells were cultured for 2 h. Proliferation was evaluated after 7 and 14 days. After 14 days, total protein content and ALP activity were measured. For bone-like nodule formation, cells were cultured for 21 days. Data were compared by ANOVA and Duncan's multiple range test when appropriate.
Cell attachment was not affected by surface topography (p=0.37). Proliferation (p=0.001), total protein content (p=0.039), ALP activity (p=0.050), and bone-like nodule formation (p=0.00001) were all significantly decreased by the most irregular surface (HA30). SIGNIFICANCE. These results suggest that initial cell events were not affected by the surface topography of the HA. However, intermediary and final events such as proliferation, protein synthesis, ALP activity, and bone-like nodule formation favored surfaces with a more regular topography, such as that presents in HA with 15% or less of microporosity.
羟基磷灰石（HA）已在骨科、牙科和颌面外科手术中用作骨替代物。
本研究旨在探讨微孔存在所产生的表面形貌对大鼠骨髓细胞反应的影响，评估指标包括：细胞附着、增殖、总蛋白含量、碱性磷酸酶（ALP）活性以及类骨结节形成。
将细胞培养在通过单轴粉末压制和不同烧结条件组合制造的HA圆盘上，这些圆盘具有不同百分比的微孔率（<5%-HA5、15%-HA15和30%-HA30）。为评估细胞附着，细胞培养2小时。在7天和14天后评估增殖情况。14天后，测量总蛋白含量和ALP活性。为观察类骨结节形成，细胞培养21天。数据在适当情况下通过方差分析和邓肯多重范围检验进行比较。
细胞附着不受表面形貌影响（p = 0.37）。最不规则的表面（HA30）使增殖（p = 0.001）、总蛋白含量（p = 0.039）、ALP活性（p = 0.050）和类骨结节形成（p = 0.00001）均显著降低。意义：这些结果表明，初始细胞事件不受HA表面形貌的影响。然而，诸如增殖、蛋白质合成、ALP活性和类骨结节形成等中间和最终事件更有利于具有更规则形貌的表面，例如微孔率为15%或更低的HA所呈现的表面。
