使用肽核酸捕获法研究人端粒DNA四链体的解折叠动力学

Kinetics of unfolding the human telomeric DNA quadruplex using a PNA trap.

作者信息

Green Jeremy J, Ying Liming, Klenerman David, Balasubramanian Shankar

机构信息

Department of Chemistry, University of Cambridge, Lensfield Road, UK.

出版信息

J Am Chem Soc. 2003 Apr 2;125(13):3763-7. doi: 10.1021/ja029149w.

DOI:10.1021/ja029149w

PMID:12656607

Abstract

The kinetics of opening of the DNA quadruplex formed by the human telomeric repeat have been investigated using real-time fluorescence resonance energy transfer (FRET) measurements with a peptide nucleic acid (PNA) trap. It has been found that this opening is zero-order with respect to PNA, indicating that the initial step is a rate-limiting internal rearrangement of the quadruplex. A study of the temperature dependence of the rate of quadruplex opening was performed and the activation energy of the process estimated to be 98 +/- 8 kJ mol(-1).

摘要

利用带有肽核酸（PNA）陷阱的实时荧光共振能量转移（FRET）测量技术，研究了由人类端粒重复序列形成的DNA四链体的打开动力学。研究发现，这种打开相对于PNA是零级反应，这表明初始步骤是四链体的限速内部重排。对四链体打开速率的温度依赖性进行了研究，该过程的活化能估计为98±8 kJ mol⁻¹。