Schröder I, Werner H, Lorenz U, Köhler H, Jenssen H L
Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(5):365-71.
A transfer factor (TF) was prepared and characterized from the leucocytes of Mantoux-positiv donors using a variety of techniques. The results allowed the following conclusions: Whilst extraction by dialysis and phenol (without precipitation) led to nearly the same positiv immunological activities, when assayed in the MEM-test, phenol extracts subjected to RNA-precipitation lost considerable activity. The residual activity was associated with the lower molecular fractions, and RNA itself has shown no activity. Residues after leucocyte dialysation have also shown considerable TF-activity when extracted by phenol. It was found that phenol extraction followed by gel filtration allowed recovery of considerable quantities of active TF, confined to several specific filtration fractions. Combination of active and inactive gel filtration fractions suggested that there existed an inhibitory factor within the extracted leucocyte preparations. This modified preparation and purification procedure may allow the preparation of TF for therapeutic purposes.
利用多种技术从结核菌素试验阳性供体的白细胞中制备并表征了一种转移因子（TF）。结果得出以下结论：虽然通过透析和苯酚提取（不沉淀）导致在MEM试验中检测时具有几乎相同的阳性免疫活性，但经过RNA沉淀的苯酚提取物失去了相当大的活性。残留活性与较低分子量部分相关，并且RNA本身没有显示出活性。白细胞透析后的残留物在用苯酚提取时也显示出相当大的TF活性。发现苯酚提取后进行凝胶过滤可回收大量活性TF，其局限于几个特定的过滤部分。活性和非活性凝胶过滤部分的组合表明，提取的白细胞制剂中存在一种抑制因子。这种改进的制备和纯化程序可能允许制备用于治疗目的的TF。
