Mueller-Eckhardt C, Ritts R E
Blut. 1976 May;32(5):353-60. doi: 10.1007/BF00996475.
Human transfer factor was prepared from normal donors with marked skin reactivity against common microbial antigens by dialysis of lymphocyte extracts versus tissue culture medium (TFmd). Several batches of TFmd were tested for their ability to specifically increase the DNA synthesis of unsensitized lymphocytes in vitro in the presence of the corresponding antigens (PPD, SKSD, Candida, Thistoplasmin). No transfer or immunologic reactivity by TFmd was observed. There was, however, a significant unspecific inhibition by TFmd of lymphocyte cultures stimulated by antigens, PHA or allogeneic cells.
人转移因子是从对常见微生物抗原有明显皮肤反应性的正常供体中制备的，方法是将淋巴细胞提取物与组织培养基（TFmd）进行透析。对几批TFmd进行了测试，以检测其在相应抗原（结核菌素纯蛋白衍生物、链球菌激酶-链道酶、白色念珠菌、荚膜组织胞浆菌素）存在的情况下，在体外特异性增加未致敏淋巴细胞DNA合成的能力。未观察到TFmd有转移或免疫反应性。然而，TFmd对抗原、植物血凝素或异基因细胞刺激的淋巴细胞培养有显著的非特异性抑制作用。
