Ashorn R G, Räsänen L, Marnela K M, Krohn K J
Clin Exp Immunol. 1979 Jul;37(1):50-7.
The effect of human transfer factor (TF) or its components L-serine and/or glycine in tuberculin (PPD), or leucoagglutinin (LA) induced leucocyte migration inhibitory factor (LIF) secretion was studied. Augmentation of LIF secretion was seen with low concentration ( = 0.078 g/l) of TF when lymphocytes were cultured in minimum essential medium for suspension cultures (MEM-S), a culture medium lacking L-serine and glycine. High concentrations (0.3125-5.0 g/l dry weight) of TF were inhibitory in MEM-S. In RPMI 1640, a culture medium containing L-serine and glycine, TF was either inhibitory or had no effect. The combination of L-serine and glycine, at concentrations equivalent or lower than the optimum of TF, had an augmenting effect on LIF secretion identical to that of TF, but no inhibition at higher concentrations was seen. The results indicate that human TF contains components which have suppressive or augmenting effects on LIF secretion in vitro. The augmenting effect may be mainly due to L-serine and glycine and thus not related to TF's activity in vivo.
研究了人转移因子（TF）或其成分L-丝氨酸和/或甘氨酸对结核菌素（PPD）或白细胞凝集素（LA）诱导的白细胞迁移抑制因子（LIF）分泌的影响。当淋巴细胞在用于悬浮培养的最低必需培养基（MEM-S，一种缺乏L-丝氨酸和甘氨酸的培养基）中培养时，低浓度（=0.078g/l）的TF可使LIF分泌增加。在MEM-S中，高浓度（0.3125 - 5.0g/l干重）的TF具有抑制作用。在含有L-丝氨酸和甘氨酸的RPMI 1640培养基中，TF要么具有抑制作用，要么没有影响。L-丝氨酸和甘氨酸的组合，在浓度等于或低于TF的最佳浓度时，对LIF分泌的增强作用与TF相同，但在较高浓度时未见抑制作用。结果表明，人TF含有对体外LIF分泌具有抑制或增强作用的成分。增强作用可能主要归因于L-丝氨酸和甘氨酸，因此与TF在体内的活性无关。
