高尔基体蛋白84是一种参与高尔基体结构形成的RAB1结合蛋白。
Golgin-84 is a rab1 binding partner involved in Golgi structure.
作者信息
Satoh Ayano, Wang Yanzhuang, Malsam Jörg, Beard Matthew B, Warren Graham
机构信息
Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, Connecticut 06520-8002, USA.
出版信息
Traffic. 2003 Mar;4(3):153-61. doi: 10.1034/j.1600-0854.2003.00103.x.
Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.
卷曲螺旋蛋白家族的高尔基体蛋白成员与囊泡拴系于高尔基体膜以及扁平囊膜之间相互连接有关。许多高尔基体蛋白还与RAB GTP酶结合。高尔基体蛋白84是一种膜锚定的高尔基体蛋白，我们现在发现它优先与RAB1 GTP酶的GTP形式结合。通过免疫电镜观察，它也存在于整个高尔基体堆栈中。针对高尔基体蛋白84的抗体在无细胞高尔基体重新组装试验中可抑制扁平囊膜的堆叠，而高尔基体蛋白84的细胞质结构域则刺激堆叠并增加重新组装堆栈的长度。在NRK细胞中瞬时表达高尔基体蛋白84有助于防止通常由布雷菲德菌素A处理引发的高尔基体解体。这些数据共同表明，高尔基体蛋白84参与了高尔基体结构的生成和维持。
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