家族性地中海热患者治疗后泪液中的秋水仙碱。
Colchicine in tear fluid of treated patients with familial Mediterranean fever.
作者信息
Leibovitch Igal, Alster Yair, Scherrmann Jean Michel, Azmon Barak, Barequet Irina S, Livneh Avi, O'Brien Terrence P, Lazar Moshe, Loewenstein Anat
机构信息
Department of Ophthalmology, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
出版信息
Cornea. 2003 Apr;22(3):191-3. doi: 10.1097/00003226-200304000-00001.
PURPOSE
The study aimed to determine whether detectable concentrations of colchicine are present in the tear fluid of treated patients with familial Mediterranean fever (FMF) and thus demonstrate a possible route by which colchicine reaches the corneal surface.
METHODS
Tear fluid samples (50-100 microL) were collected from eight FMF patients on long-term colchicine treatment. Colchicine tear fluid concentrations were determined in all patients by radioimmunoassay using goat anticolchicine antibodies and [3H]colchicine (Dupont, Wilmington, DE).
RESULTS
Detectable concentrations of colchicine, with no apparent effect on the ocular surface, were found in all tear fluid samples (median, 0.46 ng/mL; range, 0.24-1.05 ng/mL).
CONCLUSIONS
This study provides evidence of the route by which colchicine, given systemically, reaches the corneal surface and thus gives credence to the possible inhibitory effect of this drug on corneal wound healing in the cases described in the literature.
目的
本研究旨在确定接受治疗的家族性地中海热（FMF）患者的泪液中是否存在可检测浓度的秋水仙碱，从而证明秋水仙碱到达角膜表面的可能途径。
方法
从8例长期接受秋水仙碱治疗的FMF患者中采集泪液样本（50 - 100微升）。使用山羊抗秋水仙碱抗体和[3H]秋水仙碱（杜邦公司，特拉华州威尔明顿）通过放射免疫分析法测定所有患者泪液中秋水仙碱的浓度。
结果
在所有泪液样本中均发现了可检测浓度的秋水仙碱，且对眼表无明显影响（中位数为0.46纳克/毫升；范围为0.24 - 1.05纳克/毫升）。
结论
本研究提供了全身给药的秋水仙碱到达角膜表面途径的证据，从而证实了该药物对文献中所述病例角膜伤口愈合可能具有的抑制作用。
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