Fast M D, Burka J F, Johnson S C, Ross N W
National Research Council of Canada, Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada.
J Parasitol. 2003 Feb;89(1):7-13. doi: 10.1645/0022-3395(2003)089[0007:ERFLSI]2.0.CO;2.
Adult and mobile preadult sea lice Lepophtheirus salmonis were incubated with mucus samples from rainbow trout (Oncorhynchus mykiss), coho salmon (O. kisutch), Atlantic salmon (Salmo salar), and winter flounder (Pseudopleuronectes americanus) to determine the response of L. salmonis to fish skin mucus as assessed by the release of proteases and alkaline phosphatase. There was variation in the release of respective enzymes by sea lice in response to different fish. As well, sealice collected from British Columbia responded differently than New Brunswick sea lice to coho salmon mucus. Fish mucus and seawater samples were also analyzed using protease gel zymography to observe changes in the presence of low molecular weight (LMW) proteases after L. salmonis incubation. Significantly higher proportions of sea lice secreted multiple bands of L. salmonis-derived LMW proteases after incubation with rainbow trout or Atlantic salmon mucus in comparison with seawater, coho salmon, or winter flounder mucus. Susceptibility to L. salmonis infections may be related to the stimulation of LMW proteases from L. salmonis by fish mucus. The resistance of coho salmon to L. salmonis infection may be due to agents in their mucus that block the secretion of these LMW proteases or factors may exist in the mucus of susceptible species that stimulate their release.
将成年和移动性未成年海虱鲑居尾孢虫与虹鳟（Oncorhynchus mykiss）、银大麻哈鱼（O. kisutch）、大西洋鲑（Salmo salar）和美洲黄盖鲽（Pseudopleuronectes americanus）的黏液样本一起孵育，以通过蛋白酶和碱性磷酸酶的释放来确定鲑居尾孢虫对鱼皮黏液的反应。海虱对不同鱼类的反应中，各自酶的释放存在差异。此外，从不列颠哥伦比亚省采集的海虱与新不伦瑞克省的海虱对银大麻哈鱼黏液的反应不同。还使用蛋白酶凝胶酶谱法分析鱼黏液和海水样本，以观察鲑居尾孢虫孵育后低分子量（LMW）蛋白酶的存在变化。与海水、银大麻哈鱼或美洲黄盖鲽黏液相比，与虹鳟或大西洋鲑黏液孵育后，分泌多条鲑居尾孢虫来源的低分子量蛋白酶条带的海虱比例显著更高。对鲑居尾孢虫感染的易感性可能与鱼黏液对鲑居尾孢虫低分子量蛋白酶的刺激有关。银大麻哈鱼对鲑居尾孢虫感染的抵抗力可能是由于其黏液中的物质阻止了这些低分子量蛋白酶的分泌，或者易感物种的黏液中可能存在刺激其释放的因素。
