Cantón Emilia, Pemán Javier, Viudes Angel, Quindós Guillermo, Gobernado Miguel, Espinel-Ingroff Ana
Unidad de Microbiología Experimental-Centro de Investigación, Hospital Universitario La Fe, 46009, Valencia, Spain.
Diagn Microbiol Infect Dis. 2003 Mar;45(3):203-6. doi: 10.1016/s0732-8893(02)00525-4.
Minimum fungicidal concentrations (MFCs) of amphotericin B were obtained for 165 bloodstream isolates (104 Candida parapsilosis, 14 C.glabrata, 13 C.tropicalis, 15 C.krusei, and 19 C.albicans) and 36 C.dubliniensis from oropharyngeal infections. Minimum inhibitory concentrations (MICs) were determined by the M27-A microdilution method. MFCs (> or =99.9% killing) were obtained following MIC determination (inoculum size, 10(4) CFU/ml) by seeding the entire volume of all clear wells. The best fungicidal activity was for C. albicans, (MFC90 1 microg/ml) and the lowest for C.parapsilosis, C.tropicalis and C.glabrata (MFC90 16 microg/ml). Although MFCs were > or =16x MIC for some isolates, including C. glabrata, the overall MFCs were > or =2x MICs. However, major differences between MICs and MFCs were observed for C.parapsilosis and C.dubliniensis (3.8% and 8.9%, respectively, were tolerant: MFC > or =32MIC). MFCs for C.tropicalis and C. glabrata were > or =2 microg/ml. By this more stringent method we found substantial differences from those previously reported between amphotericin B MIC and MFCs for Candida spp.
测定了两性霉素B对165株血流感染分离株（104株近平滑念珠菌、14株光滑念珠菌、13株热带念珠菌、15株克柔念珠菌和19株白念珠菌）以及36株来自口咽感染的都柏林念珠菌的最低杀菌浓度（MFC）。最低抑菌浓度（MIC）采用M27 - A微量稀释法测定。在MIC测定（接种量为10⁴CFU/ml）后，通过接种所有清亮孔的全部体积获得MFC（杀菌率≥99.9%）。白念珠菌的杀菌活性最佳（MFC90为1μg/ml），近平滑念珠菌、热带念珠菌和光滑念珠菌的杀菌活性最低（MFC90为16μg/ml）。尽管对于一些分离株，包括光滑念珠菌，MFC≥16倍MIC，但总体MFC≥2倍MIC。然而，在近平滑念珠菌和都柏林念珠菌中观察到MIC和MFC之间存在显著差异（分别有3.8%和8.9%耐受：MFC≥32倍MIC）。热带念珠菌和光滑念珠菌的MFC≥2μg/ml。通过这种更严格的方法，我们发现两性霉素B对念珠菌属的MIC和MFC与先前报道的结果存在实质性差异。
