Clement D, Risterucci A M, Motamayor J C, N'Goran J, Lanaud C
Centre de coopération internationale en recherche pour le développement (CIRAD), TA 80/02, Avenue d'Agropolis, 34398 Montpellier Cedex 5, France.
Genome. 2003 Feb;46(1):103-11. doi: 10.1139/g02-118.
Quantitative trait loci (QTL) mapping for bean traits and the number of ovules per ovary was carried out in cocoa (Theobroma cacao L.) using three test-cross progenies derived from crosses between a lower Amazon Forastero male parent (Catongo) and three female parents: one upper Amazon Forastero (IMC78) and two Trinitario (DR1 and S52). RFLP (restriction fragment length polymorphism), microsatellite, and AFLP (amplified fragment lengthpolymorphism) markers were used for mapping. Between one and six QTL for bean traits (length, weight, and shape index) and one and four QTL for the number of ovules per ovary were detected using composite interval mapping (CIM). Individual QTL explained between 5 and 24% of the phenotypic variation. QTL clusters were identified on several chromosomes, but particularly on chromosome 4. QTL related to bean traits were detected in the same region in both Trinitario parents and in a close region in the upper Amazon Forastero parent. In reference to a previous diversity study where alleles specific to Criollo and Forastero genotypes were identified, it was possible to speculate on the putative origin (Criollo or Forastero) of favorable QTL alleles segregating in both Trinitario studied.
利用来自亚马逊下游弗拉斯特罗雄性亲本（卡东戈）与三个雌性亲本杂交产生的三个测交后代，对可可（Theobroma cacao L.）的豆荚性状和每个子房的胚珠数进行了数量性状基因座（QTL）定位。这三个雌性亲本分别是一个亚马逊上游弗拉斯特罗（IMC78）和两个特立尼达（DR1和S52）。采用限制性片段长度多态性（RFLP）、微卫星和扩增片段长度多态性（AFLP）标记进行定位。使用复合区间作图（CIM）检测到1至6个控制豆荚性状（长度、重量和形状指数）的QTL以及1至4个控制每个子房胚珠数的QTL。单个QTL解释了5%至24%的表型变异。在几条染色体上鉴定出了QTL簇，尤其是在第4号染色体上。与豆荚性状相关的QTL在两个特立尼达亲本的同一区域以及亚马逊上游弗拉斯特罗亲本的一个相近区域被检测到。参考之前一项鉴定了克里奥洛和弗拉斯特罗基因型特异性等位基因的多样性研究，有可能推测出在两个研究的特立尼达亲本中分离的有利QTL等位基因的假定起源（克里奥洛或弗拉斯特罗）。
