Pehlivan T, Rubio C, Rodrigo L, Romero J, Remohi J, Simón C, Pellicer A
Instituto Valenciano de Infertilidad (IVI), Plaza de la Policia Local, 3, 46015 Valencia, Spain.
Reprod Biomed Online. 2003 Mar;6(2):232-7. doi: 10.1016/s1472-6483(10)61715-4.
Implantation failure (IF) is defined as three or more failed IVF attempts, and preimplantation genetic diagnosis (PGD) is being used in these patients to improve IVF outcome. PGD was performed in 49 implantation failure patients with a mean number of 4.2 +/- 1.6 previous IVF failures, and in nine fertile controls. Fluorescence in-situ hybridization (FISH) on blastomeres from biopsied day 3 embryos was performed for chromosomes 13, 16, 18, 21, 22, X and Y. There was a significantly higher rate of chromosomal abnormalities (67.4%) compared with controls (36.3%). In 57 cycles, a pregnancy rate of 34.0% and an implantation rate of 19.8% was observed in implantation failure patients compared with controls (33.3 and 24.1% respectively), with all the pregnancies in the implantation failure group coming from the transfer of at least one chromosomally normal blastocyst on day 5. It is concluded that in IVF patients, use of PGD along with blastocyst transfer improves IVF outcome.
种植失败（IF）被定义为三次或更多次体外受精（IVF）尝试失败，目前正在对这些患者使用植入前遗传学诊断（PGD）来改善IVF结局。对49例种植失败患者进行了PGD，这些患者之前IVF失败的平均次数为4.2±1.6次，并选取了9名可育对照者。对活检的第3天胚胎的卵裂球进行13、16、18、21、22、X和Y染色体的荧光原位杂交（FISH）。与对照组（36.3%）相比，染色体异常率显著更高（67.4%）。在57个周期中，种植失败患者的妊娠率为34.0%，种植率为19.8%，而对照组分别为33.3%和24.1%，种植失败组所有妊娠均来自于第5天至少移植一枚染色体正常的囊胚。结论是，在IVF患者中，PGD与囊胚移植联合使用可改善IVF结局。
