Frary A, Doganlar S, Daunay M C, Tanksley S D
Department of Plant Breeding and Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA.
Theor Appl Genet. 2003 Jul;107(2):359-70. doi: 10.1007/s00122-003-1257-5. Epub 2003 Apr 3.
An interspecific F(2) population from a cross between cultivated eggplant, Solanum melongena, and its wild relative, S. linnaeanum, was analyzed for quantitative trait loci (QTL) affecting leaf, flower, fruit and plant traits. A total of 58 plants were genotyped for 207 restriction fragment length polymorphism (RFLP) markers and phenotyped for 18 characters. One to eight loci were detected for each trait with a total of 63 QTL identified. Overall, 46% of the QTL had allelic effects that were the reverse of those predicted from the parental phenotypes. Wild alleles that were agronomically superior to the cultivated alleles were identified for 42% of the QTL identified for flowering time, flower and fruit number, fruit set, calyx size and fruit glossiness. Comparison of the map positions of eggplant loci with those for similar traits in tomato, potato and pepper revealed that 12 of the QTL have putative orthologs in at least one of these other species and that putative orthology was most often observed between eggplant and tomato. Traits showing potential orthology were: leaf length, shape and lobing; days to flowering; number of flowers per inflorescence; plant height and apex, leaf and stem hairiness. The functionally conserved loci included a major leaf lobing QTL ( llob6.1) that is putatively orthologous to the potato leaf ( c) and/or Petroselinum ( Pts) mutants of tomato, two flowering time QTL ( dtf1.1, dtf2.1) that also have putative counterparts in tomato and four QTL for trichomes that have potential orthologs in tomato and potato. These results support the mounting evidence of conservation of gene function during the evolution of eggplant and its relatives from their last common ancestor and indicate that this conservation was not limited to domestication traits.
对栽培茄子（Solanum melongena）与其野生近缘种林奈茄（S. linnaeanum）杂交产生的种间F(2)群体进行了分析，以确定影响叶片、花朵、果实和植株性状的数量性状位点（QTL）。对58株植株进行了207个限制性片段长度多态性（RFLP）标记的基因分型，并对18个性状进行了表型分析。每个性状检测到1至8个位点，共鉴定出63个QTL。总体而言，46%的QTL等位基因效应与根据亲本表型预测的效应相反。在为开花时间、花和果实数量、坐果率、花萼大小和果实光泽度鉴定出的QTL中，42%的QTL鉴定出了在农艺学上优于栽培等位基因的野生等位基因。将茄子位点的图谱位置与番茄、马铃薯和辣椒中相似性状的图谱位置进行比较，发现其中12个QTL在至少一个其他物种中具有假定的直系同源物，并且在茄子和番茄之间最常观察到假定的直系同源关系。显示潜在直系同源关系的性状包括：叶片长度、形状和裂片；开花天数；每花序花数；株高和顶端、叶片及茎的毛被情况。功能保守的位点包括一个主要的叶片裂片QTL（llob6.1），它可能与番茄的马铃薯叶（c）和/或岩芹（Pts）突变体直系同源，两个开花时间QTL（dtf1.1、dtf2.1）在番茄中也有假定的对应物，以及四个控制毛状体的QTL，它们在番茄和马铃薯中具有潜在的直系同源物。这些结果支持了越来越多的证据，即茄子及其近缘种从它们的最后一个共同祖先进化过程中基因功能具有保守性，并且表明这种保守性并不局限于驯化性状。
