Chaturvedula V S, Schilling Jennifer K, Malone Stan, Wisse Jan H, Werkhoven Marga C, Kingston David G
Planta Med. 2003 Mar;69(3):271-4. doi: 10.1055/s-2003-38488.
Two novel triterpene acids, esculentoic acid A ( 1) and B ( 2) as well as the seven known compounds 3 - 9 were isolated from an EtOAc extract of leaves, stems, and twigs of Manihot esculenta by bioassay-guided fractionation for cytotoxic activity. The structures of the two new compounds were established as 3alpha-hydroxytaraxer-14-en-29-oic acid ( 1) and 3-oxotaraxer-14-en-29-oic acid ( 2) on the basis of 1D and 2D NMR spectroscopic data interpretation and chemical conversions. The two new compounds 1 and 2 showed moderate cytotoxicity against the A2780 human ovarian cancer cell line.
通过生物活性导向分离法，从木薯叶、茎和嫩枝的乙酸乙酯提取物中分离得到两种新型三萜酸，即香豆酸A（1）和B（2）以及七种已知化合物3 - 9，用于细胞毒性活性研究。基于一维和二维核磁共振光谱数据解析及化学转化，确定这两种新化合物的结构分别为3α-羟基蒲公英赛-14-烯-29-酸（1）和3-氧代蒲公英赛-14-烯-29-酸（2）。这两种新化合物1和2对A2780人卵巢癌细胞系显示出中等程度的细胞毒性。
