van Beilen Jan B, Duetz Wouter A, Schmid Andreas, Witholt Bernard
Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland.
Trends Biotechnol. 2003 Apr;21(4):170-7. doi: 10.1016/S0167-7799(03)00032-5.
Oxygenases carry out the regio-, stereo- and chemoselective introduction of oxygen in a tremendous range of organic molecules. This versatility has already been exploited in several commercial processes. There are, however, many hurdles to further practical large-scale applications. Here, we review various issues in biocatalysis using these enzymes, such as screening strategies, overoxidation, uncoupling, substrate uptake, substrate toxicity, and oxygen mass transfer. By addressing these issues in a systematic way, the productivity of promising laboratory scale biotransformations involving oxygenases may be improved to levels that allow industry to realise the full commercial potential of these enzymes.
加氧酶能在种类繁多的有机分子中进行区域、立体和化学选择性的氧引入反应。这种多功能性已在多个商业过程中得到应用。然而，进一步实现实际大规模应用仍存在许多障碍。在此，我们综述了使用这些酶进行生物催化时的各种问题，如筛选策略、过度氧化、解偶联、底物摄取、底物毒性和氧传质等。通过系统地解决这些问题，涉及加氧酶的有前景的实验室规模生物转化的生产率可能会提高到使工业界能够充分实现这些酶商业潜力的水平。
