荧光假单胞菌在低比生长速率下主要产生二氢形式的绿脓菌素。
Fluorescent Pseudomonas mainly produce the dihydro form of pyoverdine at low specific growth rate.
作者信息
Jacques P, Ongena M, Bernard F, Fuchs R, Budzikiewicz H, Thonart P
机构信息
Centre Wallon de Biologie Industrielle, Unité de Bioindustries, Faculté universitaire des Sciences agronomiques, B-5030 Gembloux, Belgium.
出版信息
Lett Appl Microbiol. 2003;36(5):259-62. doi: 10.1046/j.1472-765x.2003.01304.x.
AIMS
To analyse the influence of cell growth rate and iron concentration on the production of pyoverdines (PVDs) and of their reduced dihydro forms by three fluorescent Pseudomonas strains (P. putida BTP16, P. fluorescens BTP7 and P. aeruginosa 7NSK2).
METHODS
PVD and dihydropyoverdine (DHPVD) productions were determined by LC ESI-MS and spectrophotometry during batch and chemostat culture at different dilution rates.
SIGNIFICANCE
The relatively high PVD-to-DHPVD ratio (0.57) observed in pH-controlled batch cultures suggested that a base-catalysed chemical oxidation of the dihydroform is not the prime mechanism involved in generating PVDs. Interestingly, in chemostat cultures the PVD-to-DHPVD ratio was significantly reduced at low specific growth rate. Our results suggest that the oxidation of DHPVD to PVD is catalysed by an iron-dependent enzymatic reaction rather than a chemical oxidation.
目的
分析细胞生长速率和铁浓度对三种荧光假单胞菌菌株（恶臭假单胞菌BTP16、荧光假单胞菌BTP7和铜绿假单胞菌7NSK2）产生的绿脓菌素（PVDs）及其还原二氢形式的影响。
方法
在分批培养和恒化器培养中，于不同稀释率下，通过液相色谱电喷雾质谱法和分光光度法测定PVD和二氢绿脓菌素（DHPVD）的产量。
意义
在pH值控制的分批培养中观察到相对较高的PVD与DHPVD比率（0.57），这表明二氢形式的碱催化化学氧化不是产生PVDs的主要机制。有趣的是，在恒化器培养中，在低比生长速率下PVD与DHPVD比率显著降低。我们的结果表明，DHPVD氧化为PVD是由铁依赖性酶促反应催化的，而不是化学氧化。
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