宾夕法尼亚州肩突硬蜱中嗜吞噬细胞无形体和伯氏疏螺旋体的分子特征分析
Molecular characterization of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes scapularis ticks from Pennsylvania.
作者信息
Courtney Joshua W, Dryden Richard L, Montgomery Jill, Schneider Bradley S, Smith Gary, Massung Robert F
机构信息
Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1569-73. doi: 10.1128/JCM.41.4.1569-1573.2003.
Abstract
Ixodes scapularis ticks were collected in 2000 and 2001 from two areas in Pennsylvania and tested for the presence of Anaplasma phagocytophilum and Borrelia burgdorferi by PCR and DNA sequencing. Of the ticks collected from northwestern and southeastern Pennsylvania, 162 of 263 (61.6%) and 25 of 191 (13.1%), respectively, were found to be positive for B. burgdorferi. DNA sequencing showed >99% identity with B. burgdorferi strains B31 and JD1. PCR testing for A. phagocytophilum revealed that 5 of 263 (1.9%) from northwestern Pennsylvania and 76 of 191 (39.8%) from southeastern Pennsylvania were positive. DNA sequencing revealed two genotypes of A. phagocytophilum, the human granulocytic ehrlichiosis (HGE) agent and a variant (AP-Variant 1) that has not been associated with human infection. Although only the HGE agent was present in northwestern Pennsylvania, both genotypes were found in southeastern Pennsylvania. These data add to a growing body of evidence showing that AP-Variant 1 is the predominant agent in areas where both genotypes coexist.
摘要
2000年和2001年，从宾夕法尼亚州的两个地区采集了肩突硬蜱，并通过聚合酶链反应（PCR）和DNA测序检测嗜吞噬细胞无形体和伯氏疏螺旋体的存在情况。从宾夕法尼亚州西北部和东南部采集的蜱中，分别有263只中的162只（61.6%）和191只中的25只（13.1%）被检测出伯氏疏螺旋体呈阳性。DNA测序显示与伯氏疏螺旋体菌株B31和JD1的同源性>99%。对嗜吞噬细胞无形体的PCR检测显示，宾夕法尼亚州西北部263只中的5只（1.9%）和东南部191只中的76只（39.8%）呈阳性。DNA测序揭示了嗜吞噬细胞无形体的两种基因型，即人类粒细胞埃立克体病（HGE）病原体和一种尚未与人类感染相关的变体（AP - 变体1）。虽然宾夕法尼亚州西北部仅存在HGE病原体，但在东南部发现了两种基因型。这些数据进一步证明，在两种基因型共存的地区，AP - 变体1是主要病原体。
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