Cao Wu-Chun, Zhao Qiu-Min, Zhang Pan-He, Yang Hong, Wu Xiao-Ming, Wen Bo-Hai, Zhang Xi-Tan, Habbema J Dik F
Department of Epidemiology, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, People's Republic of China.
Am J Trop Med Hyg. 2003 May;68(5):547-50. doi: 10.4269/ajtmh.2003.68.547.
A total of 1,345 Ixodes persulcatus ticks collected from northeastern China were investigated for the presence of Anaplasma phagocytophila and Borrelia burgdorferi by a nested polymerase chain reaction (PCR). Sixty-two (4.6%) ticks were positive for A. phagocytophila and 454 (33.8%) were positive for B. burgdorferi. Seven (0.5%) were coinfected with both agents. Sequence analysis of 919-basepair PCR amplicons revealed three types of A. phagocytophila. Type 1 was identical to the published sequences of A. phagocytophilas responsible for human granulocytic ehrlichiosis (HGE). The other two variants differed from the HGE agent sequence at one and four positions, respectively. These findings imply that infection with A. phagocytophila poses a potential health threat to both humans and animals in northeastern China, and that ehrlichiosis should be considered in the differential diagnosis of febrile patients with a history of tick bite, particularly when clinical manifestations are atypical for Lyme disease.
采用巢式聚合酶链反应（PCR）对从中国东北地区采集的1345只全沟硬蜱进行嗜吞噬细胞无形体和伯氏疏螺旋体检测。62只蜱（4.6%）嗜吞噬细胞无形体呈阳性，454只蜱（33.8%）伯氏疏螺旋体呈阳性。7只蜱（0.5%）同时感染了这两种病原体。对919碱基对PCR扩增产物进行序列分析，发现嗜吞噬细胞无形体有三种类型。1型与已发表的引起人类粒细胞埃立克体病（HGE）的嗜吞噬细胞无形体序列相同。另外两个变体分别在一个和四个位置上与HGE病原体序列不同。这些发现表明，嗜吞噬细胞无形体感染对中国东北地区的人类和动物均构成潜在健康威胁，对于有蜱叮咬史的发热患者，尤其是临床表现不典型的莱姆病患者，在鉴别诊断时应考虑埃立克体病。
