通过高灵敏度实时荧光定量PCR对鹦鹉热衣原体和猪衣原体进行定量检测，发现牛阴道感染的患病率很高。
Quantitative detection of Chlamydia psittaci and C. pecorum by high-sensitivity real-time PCR reveals high prevalence of vaginal infection in cattle.
作者信息
DeGraves Fred J, Gao Dongya, Hehnen Hans-Robert, Schlapp Tobias, Kaltenboeck Bernhard
机构信息
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519, USA.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1726-9. doi: 10.1128/JCM.41.4.1726-1729.2003.
Abstract
Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti-Chlamydia antibodies.
摘要
通过高灵敏度、高特异性定量PCR分析牛阴道细胞刷标本中衣原体属的存在情况。在未交配的小母牛中检测到鹦鹉热衣原体和牛衣原体低水平生殖道感染的患病率为53%，这表明衣原体在牛中主要通过非生殖道传播，并且与抗衣原体抗体的高血清阳性率相符。
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