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本文引用的文献

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High-sensitivity quantitative PCR platform.高灵敏度定量PCR平台。
Biotechniques. 2003 Jan;34(1):106-10, 112-5. doi: 10.2144/03341rr01.
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Replicate PCR testing and probit analysis for detection and quantitation of Chlamydia pneumoniae in clinical specimens.用于临床标本中肺炎衣原体检测和定量的重复聚合酶链反应（PCR）检测及概率分析。
J Clin Microbiol. 2001 May;39(5):1796-801. doi: 10.1128/JCM.39.5.1796-1801.2001.
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Quantitative detection of Chlamydia spp. by fluorescent PCR in the LightCycler.在LightCycler中通过荧光PCR对衣原体属进行定量检测。
Biotechniques. 2001 Jan;30(1):150-7. doi: 10.2144/01301rr03.
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Molecular diagnosis of Chlamydia pneumoniae infection.肺炎衣原体感染的分子诊断
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Use of synthetic antigens improves detection by enzyme-linked immunosorbent assay of antibodies against abortigenic Chlamydia psittaci in ruminants.合成抗原的使用提高了通过酶联免疫吸附测定法检测反刍动物中针对流产型鹦鹉热衣原体抗体的能力。
J Clin Microbiol. 1997 Sep;35(9):2293-8. doi: 10.1128/jcm.35.9.2293-2298.1997.
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Evidence for numerous omp1 alleles of porcine Chlamydia trachomatis and novel chlamydial species obtained by PCR.通过聚合酶链反应获得的猪沙眼衣原体众多omp1等位基因及新型衣原体物种的证据。
J Clin Microbiol. 1997 Jul;35(7):1835-41. doi: 10.1128/jcm.35.7.1835-1841.1997.
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Comparison of polymerase chain reaction, direct immunofluorescence, cell culture and enzyme immunoassay for the detection of Chlamydia psittaci in bull semen.聚合酶链反应、直接免疫荧光法、细胞培养法及酶免疫测定法检测公牛精液中鹦鹉热衣原体的比较
Vet Microbiol. 1994 Dec;42(4):273-80. doi: 10.1016/0378-1135(94)90058-2.
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Chlamydial infection in animals: a review.动物衣原体感染综述
Can Vet J. 1980 Jan;21(1):2-11.
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Dominance of Chlamydia psittaci-specific IgG2 subclass in the humoral immune responses of naturally and experimentally infected cattle.鹦鹉热衣原体特异性IgG2亚类在自然感染和实验感染牛的体液免疫反应中的优势地位。
Vet Immunol Immunopathol. 1987 Jul;15(4):311-22. doi: 10.1016/0165-2427(87)90003-1.
10
Bovine chlamydial abortion: serodiagnosis by modified complement-fixation and indirect inclusion fluorescence tests and enzyme-linked immunosorbent assay.牛衣原体性流产：通过改良补体结合试验、间接包涵体荧光试验和酶联免疫吸附测定进行血清学诊断。
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通过高灵敏度实时荧光定量PCR对鹦鹉热衣原体和猪衣原体进行定量检测，发现牛阴道感染的患病率很高。

Quantitative detection of Chlamydia psittaci and C. pecorum by high-sensitivity real-time PCR reveals high prevalence of vaginal infection in cattle.

作者信息

DeGraves Fred J, Gao Dongya, Hehnen Hans-Robert, Schlapp Tobias, Kaltenboeck Bernhard

机构信息

Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519, USA.

出版信息

J Clin Microbiol. 2003 Apr;41(4):1726-9. doi: 10.1128/JCM.41.4.1726-1729.2003.

DOI:10.1128/JCM.41.4.1726-1729.2003
PMID:12682170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC153858/
Abstract

Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti-Chlamydia antibodies.

摘要

通过高灵敏度、高特异性定量PCR分析牛阴道细胞刷标本中衣原体属的存在情况。在未交配的小母牛中检测到鹦鹉热衣原体和牛衣原体低水平生殖道感染的患病率为53%，这表明衣原体在牛中主要通过非生殖道传播，并且与抗衣原体抗体的高血清阳性率相符。