一种基于荧光的聚合酶链反应方法用于鉴定A群脑膜炎球菌的评估。
Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci.
作者信息
Diggle M A, Smith K, Girvan E K, Clarke S C
机构信息
Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Glasgow, United Kingdom.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1766-8. doi: 10.1128/JCM.41.4.1766-1768.2003.
Abstract
Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.
摘要
开发了基于标准和荧光的聚合酶链反应（PCR）检测方法，通过检测mynA基因来鉴定A群脑膜炎奈瑟菌。该检测方法使用细菌培养物进行了评估，但提供了在脑膜炎球菌病期间从体液中检测mynA基因所需的灵敏度。
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