Zhu Wen, Ooi Vincent E C, Chan Paul K S, Ang Put O
Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Biochem Cell Biol. 2003 Feb;81(1):25-33. doi: 10.1139/o02-169.
Bioactivity-guided fractionation of the hot water extract from the brown alga Sargassun patens led to the isolation of a polysaccharide as an antiviral component against herpes simplex viruses which are the cause of cold sores (HSV-1) and genital herpes (HSV-2). The polysaccharide contained a sulfur group that could be present as a sulfate ester. It is thus a sulfated polysaccharide with a molecular mass of about 424 kDa, and is designated SP-2a. Gas chromatographic assay showed that the polysaccharide consisted of fucose, galactose, mannose, xylose, glucose, and galactosamine. The fucose is the major constituent sugar (35.3%), followed by galactose (18.4%). The 50% effective concentration (EC50) against HSV-2, HSV-1, and HSV-1 acyclovir resistant strain was 1.3, 5.5, and 4.1 microg/mL, respectively. The 50% cytotoxic concentration (CC50) of SP-2a on the growth of normal Vero cell line was more than 4000 microg/mL. Therefore SP-2a of S. patens may be a potent agent for treating HSV infections.
对褐藻铜藻热水提取物进行生物活性导向分离，得到一种多糖，该多糖作为抗单纯疱疹病毒的抗病毒成分，单纯疱疹病毒是唇疱疹（HSV-1）和生殖器疱疹（HSV-2）的病因。该多糖含有一个可能以硫酸酯形式存在的硫基团。因此，它是一种分子量约为424 kDa的硫酸化多糖，命名为SP-2a。气相色谱分析表明，该多糖由岩藻糖、半乳糖、甘露糖、木糖、葡萄糖和半乳糖胺组成。岩藻糖是主要的组成糖（35.3%），其次是半乳糖（18.4%）。对HSV-2、HSV-1和HSV-1阿昔洛韦耐药株的50%有效浓度（EC50）分别为1.3、5.5和4.1 μg/mL。SP-2a对正常Vero细胞系生长的50%细胞毒性浓度（CC50）超过4000 μg/mL。因此，铜藻的SP-2a可能是治疗HSV感染的有效药物。
