Bo Tao, Li Ke An, Liu Huwei
Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
J Pharm Biomed Anal. 2003 Apr 1;31(5):885-91. doi: 10.1016/s0731-7085(02)00670-2.
The seeds of Datura metel were carried aboard a retrievable satellite and exposed to space environment. The effects of space environment (weightlessness and ionizing radiation) on the contents of atropine and scopolamine in D. metel were investigated by using an effective capillary zone electrophoresis (CZE) method, which employed 50 mmol/l phosphate buffer (pH 8) containing 10% (v/v) tetrahydrofuran as the running buffer. The results showed that the contents of atropine and scopolamine varied to some extent, and the earth-control group has the lowest content of atropine. However, the variation of atropine and scopolamine contents in three groups was not obvious based on t-test. At the same time, the optimization of the separation was discussed in detail and the two compounds were completely separated within 10 min with satisfactory repeatability and calibration linearity.
白花曼陀罗种子搭载返回式卫星并暴露于太空环境。采用有效的毛细管区带电泳（CZE）方法，以含10%（v/v）四氢呋喃的50 mmol/l磷酸盐缓冲液（pH 8）作为运行缓冲液，研究太空环境（失重和电离辐射）对白花曼陀罗中阿托品和东莨菪碱含量的影响。结果表明，阿托品和东莨菪碱的含量有一定程度的变化，地面对照组阿托品含量最低。然而，基于t检验，三组中阿托品和东莨菪碱含量的变化并不明显。同时，详细讨论了分离条件的优化，两种化合物在10分钟内完全分离，具有令人满意的重复性和校准线性。
