Hamedi Y, Nateghpour M, Tan-ariya P, Tiensuwan M, Silachamroon U, Looareesuwan S
Department of Parasitology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Southeast Asian J Trop Med Public Health. 2002 Sep;33(3):512-8.
Chloroquine-resistant Plasmodium vivax is emerging in Oceania, Asia and Latin America. The drug sensitivity of P. vivax to chloroquine both in vivo and in vitro in the southern part of Iran was assessed; chloroquine-resistant Plasmodium falciparum has already been documented in this area. The in vitro sensitivity of 39 P. vivax isolates was assessed: the mean IC50 and IC90 were 189 ng/ml and 698 ng/ml blood respectively; for in vivo testing, all 39 vivax malaria patients were treated with a standard regimen of chloroquine and followed-up at 28 days: the mean parasite clearance time was 67.2 +/- 22.5 hours. The in vitro development of young parasites to mature schizonts in standard test medium was compared with that obtained in McCoy's 5A medium: no significant difference was observed. Synchronization of the blood-stage parasites was performed according to Lambros' method: the method was not suitable because it was detrimental to the parasites. A number of in vitro tests were performed using both our own laboratory-predosed microplates and WHO microplates: there was no significant difference between the results.
抗氯喹间日疟原虫正在大洋洲、亚洲和拉丁美洲出现。对伊朗南部间日疟原虫在体内和体外对氯喹的药物敏感性进行了评估；该地区已有抗氯喹恶性疟原虫的记录。评估了39株间日疟原虫分离株的体外敏感性：平均半数抑制浓度（IC50）和90%抑制浓度（IC90）分别为每毫升血液189纳克和698纳克；对于体内试验，所有39例间日疟患者接受氯喹标准治疗方案，并在28天进行随访：平均寄生虫清除时间为67.2±22.5小时。将标准试验培养基中幼龄寄生虫发育为成熟裂殖体的体外情况与在 McCoy's 5A 培养基中获得的情况进行比较：未观察到显著差异。根据 Lambros 方法对血液阶段寄生虫进行同步化处理：该方法不合适，因为它对寄生虫有害。使用我们自己实验室预先加样的微孔板和世卫组织微孔板进行了多项体外试验：结果之间没有显著差异。
