Ribeiro A A C M, Elias C F, Liberti E A, Guidi W de Lima, de Souza R R
Department of Surgery, College of Veterinary Medicine, São Paulo University, São Paulo, Brazil.
Anat Histol Embryol. 2002 Dec;31(6):344-9. doi: 10.1046/j.1439-0264.2002.00415.x.
A number of neurons of the autonomic nervous system are situated in the ganglia and can be systematically divided into pre-vertebrals, paravertebrals, intramural and para-viscerals. The celiac-mesenteric ganglion, an important pre-vertebral ganglion, is located together with the abdominal aorta and links the central nervous system to the peripheral system, participating in the coordination of peripheral reflexes and principally innervating the stomach, intestines, accessory glands (liver and pancreas). In addition, the celiac-mesenteric ganglion also contributes to the innervation of the spleen and has a role in gastrointestinal motility control. This study examined the structural and ultrastructural aspects of 40 celiac-mesenteric ganglia from domestic dogs. For light microscopy ganglia were included in paraplast and stained with haematoxylin-eosin, picrosirius, toluidine blue, Calleja's and Masson's trichrome. For examination by electron microscopy, the ganglia were submitted to cryofracture, enzyme digestion, hydrolysis and fixed in 5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4). The celiac-mesenteric ganglion was observed as a ganglionic complex composed of various ganglionic units separated by types I and III collagen fibres, predominantly unmyelinated nerve fibres and continuous capillaries. This complex is surrounded by a double-layer capsule (internal and external). The principal ganglion cells had eccentric nuclei with two nucleoli, the nucleolemma was double and presented nuclear pores. In the cytoplasm there were vesicles of the Golgi apparatus, electron-dense vacuoles, mitochondrias, smooth and granulated endoplasmic reticulum and free ribosomes. In conclusion, this ganglionic complex, in contrast to similar structures in the enteric nervous system, presents separate ganglionic units in a systematic arrangement related to the extrinsic and specific innervation of the target organs.
自主神经系统的许多神经元位于神经节中，可系统地分为椎前神经节、椎旁神经节、壁内神经节和内脏旁神经节。腹腔 - 肠系膜神经节是一个重要的椎前神经节，与腹主动脉相伴，将中枢神经系统与外周系统相连，参与外周反射的协调，主要支配胃、肠、附属腺体（肝脏和胰腺）。此外，腹腔 - 肠系膜神经节也参与脾脏的神经支配，并在胃肠运动控制中发挥作用。本研究检查了40个家犬腹腔 - 肠系膜神经节的结构和超微结构方面。对于光学显微镜检查，将神经节包埋在石蜡中，并用苏木精 - 伊红、天狼星苦味酸、甲苯胺蓝、卡列哈染色法和马森三色染色法染色。为了进行电子显微镜检查，将神经节进行冷冻断裂、酶消化、水解，并固定在0.1M磷酸盐缓冲液（pH值7.4）中的5%戊二醛中。腹腔 - 肠系膜神经节被观察为由各种神经节单位组成的神经节复合体，这些单位由I型和III型胶原纤维分隔，主要是无髓神经纤维和连续的毛细血管。这个复合体被一个双层包膜（内层和外层）包围。主要的神经节细胞有偏心核，有两个核仁，核膜是双层的，并呈现核孔。在细胞质中有高尔基体小泡、电子致密空泡、线粒体、光滑和颗粒状内质网以及游离核糖体。总之，与肠神经系统中的类似结构相比，这个神经节复合体呈现出与靶器官的外在和特定神经支配相关的系统排列的独立神经节单位。
