Kebaara Bessie, Nazarenus Tara, Taylor Rachel, Atkin Audrey L
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Curr Genet. 2003 Jun;43(3):171-7. doi: 10.1007/s00294-003-0386-3. Epub 2003 Apr 15.
The Saccharomyces cerevisiae nonsense-mediated mRNA decay (NMD) pathway targets mRNAs with premature stop codons and some wild-type mRNAs for accelerated decay. Upf1p, Upf2p and Upf3p are required for NMD. NMD-targeted mRNAs are degraded rapidly in wild-type cells and stabilized in upf1, upf2 or upf3 mutants. We report here that the relative CYH2 pre-mRNA/mRNA accumulation is enhanced in cells derived from a W303 background, compared with a variety of commonly used strains. The enhanced CYH2 pre-mRNA accumulation phenotype results from a larger difference in mRNA half-lives in the W303 strains than two previously used strains. This phenotype can be selected in crosses and is also seen in upf2 and upf3 mutants. These results suggest there are genes that influence the efficiency of NMD and that yeast strains derived from the W303 background may be useful for measurement of abundance and half-lives of low abundance, short-lived NMD substrates.
酿酒酵母中的无义介导的mRNA降解（NMD）途径会靶向带有提前终止密码子的mRNA以及一些野生型mRNA，使其加速降解。NMD需要Upf1p、Upf2p和Upf3p。在野生型细胞中，被NMD靶向的mRNA会迅速降解，而在upf1、upf2或upf3突变体中则会稳定下来。我们在此报告，与多种常用菌株相比，源自W303背景的细胞中CYH2前体mRNA/mRNA的相对积累有所增强。CYH2前体mRNA积累增强的表型是由于W303菌株中mRNA半衰期的差异比之前使用的两种菌株更大。这种表型可以在杂交中被选择出来，并且在upf2和upf3突变体中也能看到。这些结果表明存在影响NMD效率的基因，并且源自W303背景的酵母菌株可能有助于测量低丰度、短寿命的NMD底物的丰度和半衰期。
