Snoeijers Sandor S, Pérez-García Alejandro, Goosen Theo, De Wit Pierre J G M
Laboratory of Phytopathology, Department of Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.
Curr Genet. 2003 May;43(2):96-102. doi: 10.1007/s00294-003-0374-7. Epub 2003 Mar 1.
The promoter of avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum contains 12 sequences within a region of 0.6 kb that are reminiscent of the binding sequences of the GATA-type regulator involved in nitrogen utilisation of the filamentous fungi Aspergillus nidulans and Neurospora crassa. Mutational analysis of this 0.6-kb promoter region, fused to the beta-glucuronidase reporter gene, revealed that two regions, each containing two TAGATA boxes in inverted orientation and overlapping by two base pairs, are important for induction of Avr9 promoter activity in A. nidulans. Each overlapping TAGATA box differentially affected Avr9 promoter activity when shifted apart by nucleotide insertions. The other regions, which do not contain two overlapping TAGATA boxes have no, or only a limited, contribution to the inducibility of promoter activity.
番茄真菌病原体fulvum的无毒基因Avr9的启动子在0.6 kb的区域内包含12个序列，这些序列让人联想到参与丝状真菌构巢曲霉和粗糙脉孢菌氮利用的GATA型调节因子的结合序列。将该0.6 kb启动子区域与β-葡萄糖醛酸酶报告基因融合进行突变分析，结果表明，两个区域对构巢曲霉中Avr9启动子活性的诱导很重要，每个区域都包含两个反向排列且有两个碱基对重叠的TAGATA框。当通过核苷酸插入使每个重叠的TAGATA框分开时，它们对Avr9启动子活性有不同的影响。其他不包含两个重叠TAGATA框的区域对启动子活性的诱导没有贡献或只有有限的贡献。
