Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
N Biotechnol. 2011 Oct;28(6):719-24. doi: 10.1016/j.nbt.2011.01.007. Epub 2011 Feb 17.
High-yield production of polyhydroxyalkanoates (PHAs) by Ralstonia eutropha KCTC 2662 was investigated using soybean oil and γ-butyrolactone as carbon sources. In flask culture, it was shown that R. eutropha KCTC 2662 accumulated PHAs during the growth phase. The optimum carbon to nitrogen ratio (C/N ratio) giving the highest cell and PHA yield was 20 g-soybean oil/g-(NH(4))(2)SO(4). The 4-hydroxybutyrate (4HB) fraction in the copolymer was not strongly affected by the C/N ratio. In a 2.5-L fermentor, a homopolymer of poly(3-hydroxybutyrate) [P(3HB)] was produced from soybean oil as the sole carbon source by batch and fed-batch cultures of R. eutropha with dry cell weights of 15-32 g/L, PHA contents of 78-83 wt% and yields of 0.80-0.82 g-PHA/g-soybean oil used. By co-feeding soybean oil and γ-butyrolactone as carbon sources, a copolymer of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] could be produced with dry cell weights of 10-21 g/L, yields of 0.45-0.56 g-PHA/g-soybean oil used (0.39-0.50g-PHA/g-carbon sources used) and 4HB fractions of 6-10 mol%. Higher supplementation of γ-butyrolactone increased the 4HB fraction in the copolymer, but decreased cell and PHA yield.
利用大豆油和γ-丁内酯作为碳源，研究了恶臭假单胞菌 KCTC 2662 高产聚羟基烷酸酯（PHA）。在摇瓶培养中，表明恶臭假单胞菌 KCTC 2662 在生长阶段积累 PHA。给出最高细胞和 PHA 产率的最佳碳氮比（C/N 比）为 20 g-大豆油/g-（NH 4 ） 2 SO 4 。共聚物中 4-羟基丁酸（4HB）的比例不受 C/N 比的强烈影响。在 2.5-L 发酵罐中，通过分批和补料分批培养恶臭假单胞菌，以大豆油为唯一碳源生产聚（3-羟基丁酸酯）[P（3HB）]均聚物，细胞干重为 15-32 g/L，PHA 含量为 78-83wt%，所用大豆油的产率为 0.80-0.82 g-PHA/g-大豆油。通过共喂养大豆油和γ-丁内酯作为碳源，可以生产聚（3-羟基丁酸-co-4-羟基丁酸酯）[P（3HB-co-4HB）]共聚物，细胞干重为 10-21 g/L，所用大豆油的产率为 0.45-0.56 g-PHA/g-大豆油（0.39-0.50g-PHA/g-碳源）和 4HB 分数为 6-10mol%。较高的γ-丁内酯补料增加了共聚物中的 4HB 分数，但降低了细胞和 PHA 的产率。
