Vedbrat S S, Whitt G S
Genetics. 1976 Mar 25;82(3):451-66. doi: 10.1093/genetics/82.3.451.
Although anopheline mosquitoes are important vectors of malaria, their genetic makeup has not yet been extensively investigated. The present studies concentrate on the genetic basis of esterases in Anopheles albinomanus. Nine zones of esterase activity activity have been resolved by gel electrophoresis. Four of these esterases: EST-2, EST-4, EST-6, and EST-8 are present throughout all developmental stages and also posess allelic variation. Mass matings were carried out with homozygous males and females heterozygous for two or more loci. The analyses of the progeny from single egg batches revealed that the four esterase systems mentioned above are encoded in separate loci with codominant allels. Analyses of two-point and three-point crosses have indicated the following linkage relationships: Est-8--12%--Est-4--22%--Est-2--9%--Est-6. The assignment of this linkage group to chromosome 3 has been accomplished by the use of a Y-2 chromosome translocation.
尽管按蚊是疟疾的重要传播媒介,但其基因组成尚未得到广泛研究。目前的研究集中在白纹按蚊酯酶的遗传基础上。通过凝胶电泳已分辨出九个酯酶活性区域。其中四种酯酶:EST-2、EST-4、EST-6和EST-8在所有发育阶段均存在,并且也具有等位基因变异。对两个或多个位点杂合的纯合雄性和雌性进行大量交配。对单个卵批次后代的分析表明,上述四种酯酶系统由具有共显性等位基因的独立位点编码。两点和三点杂交分析表明了以下连锁关系:Est-8--12%--Est-4--22%--Est-2--9%--Est-6。通过使用Y-2染色体易位已完成该连锁群在3号染色体上的定位。