Vernick K D, Collins F H, Seeley D C, Gwadz R W, Miller L H
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Biochem Genet. 1988 Jun;26(5-6):367-79. doi: 10.1007/BF02401790.
The main polymorphic system of esterase isoenzymes in adults of the G3 laboratory strain of Anopheles gambiae consists of two to five major bands of activity per individual. The bands are designated 5S, 5F, 13, 14, and 15. In genetic crosses, the genes which coded for the bands assorted as three codominant alleles, Est A, Est B, and Est C, at a single autosomal locus. Homozygotes for the Est C allele were significantly underrepresented among backcross progeny. The developmental pattern of esterase expression was examined. Esterase gene expression in embryos was first detectable between 2 and 12 hr after oviposition. The initiation or termination of expression of some of the bands corresponded to boundaries between developmental stages. Most of the esterase fractions were not specifically localized within the tissues tested, with the exception of a series of bands which were restricted largely to adult male testes.
冈比亚按蚊G3实验室品系成虫酯酶同工酶的主要多态系统由每个个体两到五条主要活性带组成。这些带被命名为5S、5F、13、14和15。在遗传杂交中，编码这些带的基因在一个常染色体位点上作为三个共显性等位基因Est A、Est B和Est C进行分离。Est C等位基因的纯合子在回交后代中明显较少。研究了酯酶表达的发育模式。酯酶基因在产卵后2至12小时之间首次在胚胎中被检测到。一些带的表达起始或终止与发育阶段之间的界限相对应。除了一系列主要局限于成年雄性睾丸的带之外，大多数酯酶组分在测试组织中没有特异性定位。
