Boe-Hansen R, Martiny A C, Arvin E, Albrechtsen H J
Environment and Resources DTU, Building 115, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Water Sci Technol. 2003;47(5):91-7.
In this study, the construction a model distribution system suitable for studies of attached and suspended microbial consisted of two loops connected in series with a total of 140 biofilm sampling points. The biofilm from the system was studied using 11 different microbial methods and the results were compared and discussed. The methods were used for biomass quantification (AODC, HPC and ATP determination), visualisation of structure (CLSM), activity measurement (leucine incorporation, AOC removal rate, respiration of benzoic acid, CTC and live/dead stains), and microbial diversity profiling (clone libraries and DGGE).
在本研究中，构建了一个适用于附着和悬浮微生物研究的模型分布系统，该系统由两个串联的回路组成，共有140个生物膜采样点。使用11种不同的微生物方法对该系统中的生物膜进行了研究，并对结果进行了比较和讨论。这些方法用于生物量定量（AODC、HPC和ATP测定）、结构可视化（CLSM）、活性测量（亮氨酸掺入、AOC去除率、苯甲酸呼吸、CTC和活/死染色）以及微生物多样性分析（克隆文库和DGGE）。
