Howard R R, Fasano C S, Frey L, Miller C H
Department of Pathology, University of California Medical Center, Davis at Sacramento, USA.
Cytometry. 1996 Sep 15;26(3):231-2. doi: 10.1002/(SICI)1097-0320(19960915)26:3<231::AID-CYTO9>3.0.CO;2-H.
A study to determine reference intervals in normal Asian and non-Asian populations for CD3 (T cells), CD4 (T helper/inducer cells), CD8 (T suppressor/cytotoxic cells), and for the CD4/CD8 ratio and absolute CD4 lymphocyte count was performed. Coulter Clone/Cytostat reagents were used. The samples were lysed using the Coulter Q-Prep and analyzed on the Coulter Profile II flow cytometer. The Asian population appeared to have a lower mean percentage of CD3 and CD4 cells, a lower CD4/CD8 ratio, and lower absolute CD4 lymphocytes. These findings indicate that race should be a consideration in determining reference intervals for immunophenotyping for CD3, CD4, and CD8.
开展了一项研究，以确定正常亚洲和非亚洲人群中CD3（T细胞）、CD4（T辅助/诱导细胞）、CD8（T抑制/细胞毒性细胞）、CD4/CD8比值及CD4淋巴细胞绝对计数的参考区间。使用了库尔特克隆/细胞抑制剂试剂。样本用库尔特Q-Prep进行裂解，并在库尔特Profile II流式细胞仪上进行分析。亚洲人群的CD3和CD4细胞平均百分比、CD4/CD8比值及CD4淋巴细胞绝对计数似乎较低。这些发现表明，在确定CD3、CD4和CD8免疫表型分析的参考区间时应考虑种族因素。
