Wilkins T D, Chalgren S L, Jimenez-Ulate F, Drake C R, Johnson J L
J Clin Microbiol. 1976 Mar;3(3):359-63. doi: 10.1128/jcm.3.3.359-363.1976.
Bacteroides fragilis strains formed much smaller colonies on most types of blood agar plates than they did on the same media without blood. Blood inhibited strains of B. fragilis subsp. distasonis the most and B. fragilis subsp. fragilis the least. The inhibition could be eliminated by adding hemin to the blood agar. The inhibitory component of the blood was inside the erythrocytes and appeared to be the hemin-free globin of hemoglobin.
脆弱拟杆菌菌株在大多数类型的血琼脂平板上形成的菌落比在不含血液的相同培养基上形成的菌落小得多。血液对脆弱拟杆菌亚种狄氏拟杆菌的抑制作用最强,对脆弱拟杆菌亚种脆弱拟杆菌的抑制作用最弱。通过向血琼脂中添加血红素可以消除这种抑制作用。血液中的抑制成分存在于红细胞内,似乎是血红蛋白中不含血红素的珠蛋白。