厌氧细菌临床分离株在琼脂培养基上的生长：培养基成分、储存条件及厌氧条件下还原作用的影响
Growth of clinical isolates of anaerobic bacteria on agar media: effects of media composition, storage conditions, and reduction under anaerobic conditions.
作者信息
Murray P R
出版信息
J Clin Microbiol. 1978 Dec;8(6):708-14. doi: 10.1128/jcm.8.6.708-714.1978.
Abstract
The quantitative growth, the colony size, and the rate of growth of 47 clinical anaerobic isolates were compared on five different media, namely Brucella agar, brain heart infusion agar, Columbia agar, Schaedler agar, and tryptic soy agar. There was no significant difference in the quantitative growth of the anaerobes inoculated onto the five media. Although no single medium was superior for the growth of all isolates, 12 of 22 isolates, inoculated onto media stored for 4 weeks or less, grew best on Schaedler agar. The effects of supplementation of the media with reducing agents and reduction of the media before use were also analyzed and were found to be affected by the composition and length of storage of the media, as well as the bacteria tested.
摘要
比较了47株临床厌氧分离株在五种不同培养基上的定量生长、菌落大小和生长速率，这五种培养基分别是布鲁氏菌琼脂、脑心浸液琼脂、哥伦比亚琼脂、 Schaedler琼脂和胰蛋白胨大豆琼脂。接种到这五种培养基上的厌氧菌定量生长没有显著差异。虽然没有一种单一的培养基对所有分离株的生长都是最优的，但接种到储存4周或更短时间的培养基上的22株分离株中有12株在Schaedler琼脂上生长最好。还分析了在培养基中添加还原剂以及在使用前对培养基进行还原的效果，发现这些效果受培养基的组成、储存时间以及所测试细菌的影响。
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